Scholarlink

Background <p>Demineralized dentin matrix (DDM) can serve as a novel scaffold for dental tissue regeneration. This study aimed to assess the impact of two distinct sterilizing methods on morphology of Allo-DDM as well as on the viability, metabolic activity, and alkaline phosphatase (ALP) activity of bone marrow mesenchymal stem cells (BMMSCs).</p> Methods <p>Allo-DDM scaffolds were prepared from human-extracted premolars and sterilized using two different techniques. These scaffolds were examined using scanning electron microscopy (SEM) to assess their morphology and by Energy Dispersive X-ray Spectroscopy (EDAX) to assess the scaffolds’ mineral content. BMMSCs were grouped into four groups: [<CitationRef CitationID="CR1">1</CitationRef>] Collagen group (positive control): Stem cells received a collagen scaffold; [<CitationRef CitationID="CR2">2</CitationRef>] Gamma-ray group(G-DDM): Stem cells received gamma-sterilized DDM scaffold; [<CitationRef CitationID="CR3">3</CitationRef>] Autoclave group(A-DDM): Stem cells received autoclave-sterilized DDM scaffold), and [<CitationRef CitationID="CR4">4</CitationRef>] Negative Control group: Stem cells were provided with culture media only. Cell viability and metabolic activity were assessed using trypan blue and MTT assays, while cell differentiation was evaluated using the alkaline phosphatase (ALP) assay.</p> Results <p>SEM micrographs revealed that G-DDM had a higher number of dentinal tubules than the A-DDM group, while there were no significant differences between G-DDM and A-DDM in the EDAX analysis. Cell viability and metabolic activity assessment showed that the collagen group had significantly higher viability, followed by G-DDM, while A-DDM and negative control groups did not improve BMMSC viability metabolic activity after 48&#xa0;h. The ALP assessment revealed that cells significantly differentiated in collagen and G-DDM groups than in A-DMM and control groups.</p> Conclusion <p>Gamma sterilization more effectively preserved the structural, chemical, and biological characteristics of Allo-DDM scaffolds compared to autoclaving. Gamma-treated scaffolds outperformed autoclaved scaffolds in terms of cell survival, metabolic activity, and ALP activity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In vitro evaluation of gamma irradiation versus autoclaving on the morphology and regenerative potential of Allo-demineralized dentin matrix

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月10日

查看全文
  • Marwa Ahmed Abozed,
  • Iman Fathy,
  • Mariem Osama Wassel,
  • Wael Hamdy El shater,
  • Nagwa Mohamed Ali Khattab

摘要

Background

Demineralized dentin matrix (DDM) can serve as a novel scaffold for dental tissue regeneration. This study aimed to assess the impact of two distinct sterilizing methods on morphology of Allo-DDM as well as on the viability, metabolic activity, and alkaline phosphatase (ALP) activity of bone marrow mesenchymal stem cells (BMMSCs).

Methods

Allo-DDM scaffolds were prepared from human-extracted premolars and sterilized using two different techniques. These scaffolds were examined using scanning electron microscopy (SEM) to assess their morphology and by Energy Dispersive X-ray Spectroscopy (EDAX) to assess the scaffolds’ mineral content. BMMSCs were grouped into four groups: [1] Collagen group (positive control): Stem cells received a collagen scaffold; [2] Gamma-ray group(G-DDM): Stem cells received gamma-sterilized DDM scaffold; [3] Autoclave group(A-DDM): Stem cells received autoclave-sterilized DDM scaffold), and [4] Negative Control group: Stem cells were provided with culture media only. Cell viability and metabolic activity were assessed using trypan blue and MTT assays, while cell differentiation was evaluated using the alkaline phosphatase (ALP) assay.

Results

SEM micrographs revealed that G-DDM had a higher number of dentinal tubules than the A-DDM group, while there were no significant differences between G-DDM and A-DDM in the EDAX analysis. Cell viability and metabolic activity assessment showed that the collagen group had significantly higher viability, followed by G-DDM, while A-DDM and negative control groups did not improve BMMSC viability metabolic activity after 48 h. The ALP assessment revealed that cells significantly differentiated in collagen and G-DDM groups than in A-DMM and control groups.

Conclusion

Gamma sterilization more effectively preserved the structural, chemical, and biological characteristics of Allo-DDM scaffolds compared to autoclaving. Gamma-treated scaffolds outperformed autoclaved scaffolds in terms of cell survival, metabolic activity, and ALP activity.