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Background <p>To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of <i>Streptococcus mutans</i>, a key cariogenic pathogen in dental caries.</p> Methods <p>SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support <i>S. mutans</i> growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of <i>S. mutans</i> lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples and confirming <i>S. mutans</i> colonies via 16S rRNA sequencing.</p> Results <p>SB-10 supported robust <i>S. mutans</i> growth with larger colonies and distinctive sugar particle formation compared to MSB-20. GtfB and GtfC were essential for sugar particle deposition around colonies. SB-10 demonstrated high specificity by suppressing the growth of non-target oral bacteria and accurately isolating <i>S. mutans</i> from clinical saliva samples, with all colonies confirmed as <i>S. mutans</i> via 16S rRNA sequencing.</p> Conclusions <p>SB-10 is a novel selective medium that enhances the isolation and identification of <i>S. mutans</i>, characterized by unique sugar particles deposition and colony morphology.</p>

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A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月16日

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  • Yuwen Fang,
  • Qizhao Ma,
  • Wei Zhao,
  • Nian Liu,
  • Lei Cheng,
  • Jing Chen,
  • Jing Zou

摘要

Background

To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries.

Methods

SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support S. mutans growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of S. mutans lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples and confirming S. mutans colonies via 16S rRNA sequencing.

Results

SB-10 supported robust S. mutans growth with larger colonies and distinctive sugar particle formation compared to MSB-20. GtfB and GtfC were essential for sugar particle deposition around colonies. SB-10 demonstrated high specificity by suppressing the growth of non-target oral bacteria and accurately isolating S. mutans from clinical saliva samples, with all colonies confirmed as S. mutans via 16S rRNA sequencing.

Conclusions

SB-10 is a novel selective medium that enhances the isolation and identification of S. mutans, characterized by unique sugar particles deposition and colony morphology.