Background <p>Proper self-care practices are essential for maintaining optimal glycemic control and preventing complications related to type 2 diabetes Mellitus. This study aimed to assess the effectiveness of print-based educational intervention on self-care practices among patients with Type 2 diabetes at tertiary hospitals South-west, Nigeria.</p> Methods <p>A quasi-experimental pretest-posttest research design was employed. The sample size was 192 patients attending endocrinology clinic who were selected using purposive sampling technique and were randomly divided into print based instructional module group (<i>n</i> = 96), and control (<i>n</i> = 96). Data collection tools were demographic information questionnaire and self-care practice questionnaire. The educational intervention in the print based instructional group included power point presentation session with take home leaflet and weekly activities reminder on platforms for 12 weeks with no intervention in the control group. Data was analyzed using SPSS 26 at a significance level of 0.05.</p> Results <p>There was increase in the proportion of participants with good practice of self-care (90.4%) in the Print-Based Instructional Modules (PBIM) group compared to control group at post intervention. After the educational interventions, there were statistically significant changes observed in respondents’ practice of self-care at post-intervention in the PBIM group (10.573 to 12.957), (z=-6.845, <i>p</i> &lt; 0.001). However, there was no significant difference in the practice of self-care in the control group, (z=-0.160, <i>p</i> = 0.873).</p> Conclusions <p>Print based instructional module is effective in enhancing self-care practice among patients with type 2 diabetes. It is recommended to use a print-based instructional modules in form of leaflet to supplement routine diabetes education.</p>