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<p>Exopolysaccharide (EPS) synthesis of halophilic microorganisms is often reported as an adaptation to high-salt environments, and the unique structural and functional characteristics of these EPSs make them valuable biomaterials with promising application potential. A moderately halophilic strain, <i>Vreelandella</i> sp. DT-Z4, was isolated from Qinghai saline soil, and its EPS yield reached 3.09&#xa0;g/L after fermentation optimization. The polysaccharide fraction (designated EPS-Z4) was partially purified using ethanol precipitation, deproteinization, and dialysis processes, and subsequently characterized for monosaccharide composition, spectroscopic features, and morphological properties. Analytical results revealed that EPS-Z4 was a fructose-rich polysaccharide composed of 97.6% fructose, 1.4% glucose, and 0.5% uronic acid. Functional group analysis confirmed the presence of absorption peaks characteristic of polysaccharides. The polysaccharide showed a degradation temperature (Td = 275.9&#xa0;°C), water solubility (85.8%), and oil-holding capacity (321%). Morphological observations revealed a dense, layered network structure. Based on these physicochemical properties, the fructose-rich polysaccharide fraction EPS-Z4 may merit further investigation as a stabilizer in high-temperature food processing or as a biological agent for the remediation of saline-alkali soils.</p>

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Production and partial characterization of fructose-rich exopolysaccharide from a newly isolated halophilic strain Vreelandella sp. DT-Z4

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月24日

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  • Fengqian Yang,
  • Fangyan Wang,
  • Chengyang Wang,
  • Longzhan Gan,
  • Chunbo Dong,
  • Zhongshun Xu,
  • Xiao Zou

摘要

Exopolysaccharide (EPS) synthesis of halophilic microorganisms is often reported as an adaptation to high-salt environments, and the unique structural and functional characteristics of these EPSs make them valuable biomaterials with promising application potential. A moderately halophilic strain, Vreelandella sp. DT-Z4, was isolated from Qinghai saline soil, and its EPS yield reached 3.09 g/L after fermentation optimization. The polysaccharide fraction (designated EPS-Z4) was partially purified using ethanol precipitation, deproteinization, and dialysis processes, and subsequently characterized for monosaccharide composition, spectroscopic features, and morphological properties. Analytical results revealed that EPS-Z4 was a fructose-rich polysaccharide composed of 97.6% fructose, 1.4% glucose, and 0.5% uronic acid. Functional group analysis confirmed the presence of absorption peaks characteristic of polysaccharides. The polysaccharide showed a degradation temperature (Td = 275.9 °C), water solubility (85.8%), and oil-holding capacity (321%). Morphological observations revealed a dense, layered network structure. Based on these physicochemical properties, the fructose-rich polysaccharide fraction EPS-Z4 may merit further investigation as a stabilizer in high-temperature food processing or as a biological agent for the remediation of saline-alkali soils.