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Background <p>The rise in nosocomial infections caused by multidrug-resistant bacteria and the ability of bacteria to form biofilms have necessitated the exploration of alternative antimicrobial strategies. In this study, silver nanoparticles (AgNPs) were synthesised using a green method in the presence of curcumin to suppress the biofilm-forming abilities of Methicillin-resistant <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>.</p> Methods <p>Curcumin was utilized in a green synthesis approach to facilitate reduction and create stable AgNPs. The resulting curcumin-capped AgNPs (Cur-AgNPs) were tested for their antibacterial and antibiofilm properties using microbroth dilution and microtiter plate methods (MTP). The MTT assay was carried out to evaluate the potential toxicity of Cur-AgNPs.</p> Results <p>Cur-AgNPs exhibited a minimum inhibitory concentration (MIC) of 14.12&#xa0;µg/mL against both <i>P. aeruginosa</i> and MRSA. Furthermore, Cur-AgNPs demonstrated antibiofilm activity by suppressing biofilm formation and disrupting existing biofilms, and were non-toxic to the murine fibroblast cell line (L929) at the MIC concentration, as determined by the MTT assay.</p> Conclusion <p>Cur-AgNPs demonstrated significant antibacterial and antibiofilm activity against both Gram-positive and Gram-negative bacterial strains. These results suggest that green-synthesised AgNPs represent a promising approach for biofilm management. Furthermore, this study highlights the potential of curcumin as a capping agent to substantially improve the antibacterial and antibiofilm properties of AgNPs.</p>

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Antibacterial and antibiofilm activities of curcumin-mediated green-synthesised silver nanoparticles against Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月24日

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  • Raha Zare Shahraki,
  • Leili Shokoohizadeh,
  • Zahra Chegini,
  • Esmaeel Sharifi,
  • Mohammad Reza Arabestani

摘要

Background

The rise in nosocomial infections caused by multidrug-resistant bacteria and the ability of bacteria to form biofilms have necessitated the exploration of alternative antimicrobial strategies. In this study, silver nanoparticles (AgNPs) were synthesised using a green method in the presence of curcumin to suppress the biofilm-forming abilities of Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa.

Methods

Curcumin was utilized in a green synthesis approach to facilitate reduction and create stable AgNPs. The resulting curcumin-capped AgNPs (Cur-AgNPs) were tested for their antibacterial and antibiofilm properties using microbroth dilution and microtiter plate methods (MTP). The MTT assay was carried out to evaluate the potential toxicity of Cur-AgNPs.

Results

Cur-AgNPs exhibited a minimum inhibitory concentration (MIC) of 14.12 µg/mL against both P. aeruginosa and MRSA. Furthermore, Cur-AgNPs demonstrated antibiofilm activity by suppressing biofilm formation and disrupting existing biofilms, and were non-toxic to the murine fibroblast cell line (L929) at the MIC concentration, as determined by the MTT assay.

Conclusion

Cur-AgNPs demonstrated significant antibacterial and antibiofilm activity against both Gram-positive and Gram-negative bacterial strains. These results suggest that green-synthesised AgNPs represent a promising approach for biofilm management. Furthermore, this study highlights the potential of curcumin as a capping agent to substantially improve the antibacterial and antibiofilm properties of AgNPs.