Scholarlink

Background <p>This study aimed to determine whether <i>Mycoplasma (M) genitalium</i>, <i>M. hominis</i>, <i>Ureaplasma (U) urealyticum</i>, and <i>U. parvum</i> were present in male patients with symptoms of urethritis.</p> Methods <p>First-void urine and genital discharge samples were collected from 94 men. The samples were examined for the presence of <i>M. genitalium</i>, <i>M. hominis</i>,<i> U. urealyticum</i>, and <i>U. parvum</i> using a multiplex polymerase chain reaction (PCR) method (BioGX Mycoplasma-Ureaplasma-OSR commercial multiplex PCR kit, BioGX, the Netherlands). The presence of <i>Trichomonas vaginalis</i> and <i>Neisseria (N) gonorrhoeae</i> was investigated using microscopy and culture methods. In the <i>M. genitalium</i>–positive samples, macrolide resistance was evaluated using the Macrolide-R/MG ELITe MGB Kit (ELITechGroup S.p.A., Italy) on the ELITe BeGenius system.</p> Results <p>A total of 55 microorganisms were detected in 41.5% of the patients (<i>n</i> = 39; <i>U. urealyticum</i> [<i>n</i> = 18], <i>U. parvum</i> [<i>n</i> = 14], <i>N. gonorrhoeae</i> [<i>n</i> = 9], <i>M. hominis</i> [<i>n</i> = 8], and <i>M. genitalium</i> [<i>n</i> = 6]). The positivity rates of <i>M. genitalium</i> and <i>N. gonorrhoeae</i> were statistically higher in the patients with more than 3 partners in the last 12 months, and those of <i>U. urealyticum</i> and <i>N. gonorrhoeae</i> were higher in the patients with genital discharge (<i>p</i> &lt; 0.05). In addition, a significant relationship was found between <i>N. gonorrhoeae</i> positivity and genital itching and pain/discomfort during sexual intercourse (<i>p</i> &lt; 0.05). Macrolide resistance was detected in 2 (33.3%) of the 6 <i>M. genitalium</i>–positive samples.</p> Conclusion <p>In this study, <i>U. urealyticum</i>, <i>N. gonorrhoeae</i>, and <i>M. genitalium</i> were detected in 19.1%, 9.6%, and 6.4% of the male patients who presented with symptoms of urethritis, respectively.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Investigation of mycoplasma and ureaplasma species using a molecular method in male patients suffering from urethritis symptoms: a cross-sectional study in the city of Antalya

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月19日

查看全文
  • Tuncer Karpuz,
  • Murat Şambel,
  • Özlem Koyuncu Özyurt,
  • Ahmet Ender Caylan,
  • Ömer Kutlu,
  • Meral Dilara Öğünç,
  • Gözde Öngüt,
  • Betil Özhak,
  • Burak Saygılı,
  • Dilek Çolak,
  • Hatice Yazısız

摘要

Background

This study aimed to determine whether Mycoplasma (M) genitalium, M. hominis, Ureaplasma (U) urealyticum, and U. parvum were present in male patients with symptoms of urethritis.

Methods

First-void urine and genital discharge samples were collected from 94 men. The samples were examined for the presence of M. genitalium, M. hominis, U. urealyticum, and U. parvum using a multiplex polymerase chain reaction (PCR) method (BioGX Mycoplasma-Ureaplasma-OSR commercial multiplex PCR kit, BioGX, the Netherlands). The presence of Trichomonas vaginalis and Neisseria (N) gonorrhoeae was investigated using microscopy and culture methods. In the M. genitalium–positive samples, macrolide resistance was evaluated using the Macrolide-R/MG ELITe MGB Kit (ELITechGroup S.p.A., Italy) on the ELITe BeGenius system.

Results

A total of 55 microorganisms were detected in 41.5% of the patients (n = 39; U. urealyticum [n = 18], U. parvum [n = 14], N. gonorrhoeae [n = 9], M. hominis [n = 8], and M. genitalium [n = 6]). The positivity rates of M. genitalium and N. gonorrhoeae were statistically higher in the patients with more than 3 partners in the last 12 months, and those of U. urealyticum and N. gonorrhoeae were higher in the patients with genital discharge (p < 0.05). In addition, a significant relationship was found between N. gonorrhoeae positivity and genital itching and pain/discomfort during sexual intercourse (p < 0.05). Macrolide resistance was detected in 2 (33.3%) of the 6 M. genitalium–positive samples.

Conclusion

In this study, U. urealyticum, N. gonorrhoeae, and M. genitalium were detected in 19.1%, 9.6%, and 6.4% of the male patients who presented with symptoms of urethritis, respectively.