Background <p>Animal models are crucial for developing treatments for knee injuries. This study compared knee joint anatomy and arthroscopic surgery feasibility among turkeys, canines, and rabbits.</p> Methods <p>Knee samples from cadavers of turkeys, canines, and rabbits (<i>n</i> = 18 per group) were examined to compare anatomical, histological, and biomechanical characteristics across species. Detailed measurements were taken of the bony structures, ligaments, and menisci, while histological analyses focused on the meniscus and cartilage. Biomechanical testing of the meniscus was also conducted. Additionally, the feasibility of performing diagnostic arthroscopy, cranial cruciate ligament transection, and total medial meniscectomy was assessed and compared between species and surgeons.</p> Results <p>Significant differences were observed in various anatomical structures among the three species, with turkeys having a significantly wider patella and a different cruciate ligament orientation. Histological analysis showed substantial differences in meniscus structure and cartilage thickness between species. However, the biomechanical properties of the meniscus were fairly comparable across species. There was no significant difference in surgical time among surgeons performing these arthroscopic procedures. Nevertheless, surgery took significantly longer in rabbits than in turkeys or canines, while no significant difference in duration was found between turkeys and canines.</p> Conclusions <p>This study&#xa0;demonstrates the anatomical, histological, and biomechanical properties, as well as the feasibility of arthroscopic surgery, in three potential animal models of the knee joint. Results suggest the turkey may serve as a promising bipedal animal model for investigational arthroscopic knee surgery and research on associated traumatic knee injuries. However, anatomical differences from the human knee require careful evaluation of its suitability as a simulator before research.</p>