Scholarlink

Background <p>Neonatal sepsis remains a major global health challenge due to its contribution to infant morbidity and mortality. Early diagnosis is often difficult because the clinical presentation is nonspecific. Despite extensive research, no biomarker has yet achieved the required sensitivity, specificity and minimal blood volume to avoid hospital-acquired anaemia in newborns. Identifying a reliable early marker could improve therapeutic decision making and reduce unnecessary antibiotics use in neonates with suspected infection.</p> Methodology <p>We conducted a hospital-based comparative case-control study in the special care baby units of two tertiary hospitals in Oyo state, Nigeria, between November 2018 and March 2019. A total of 120 neonates, aged 0–72&#xa0;h were enrolled: 65 with suspected sepsis and 55 apparently healthy controls. Neutrophil CD64 expression was assessed using flow cytometry, Full Blood Count with differential and blood culture were also evaluated for every participant. Data were analyzed with SPSS Version 21 and statistical significance was set at <i>P</i> &lt; 0.05.</p> Results <p>Neonates with suspected sepsis demonstrated significantly higher white blood cell counts and band neutrophil percentages than apparently healthy controls. The neutrophil CD64 expression was significantly higher in the sepsis group. Receiver operating characteristic analysis showed that neutrophil CD64 had an area under the curve (AUC) of 0.894 with sensitivity of 93.8% and specificity of 88.1% at cut-off value of 125.53. Percentage neutrophil CD64 demonstrated an AUC of 0.887 with a sensitivity of 93.8% and specificity of 88.1% at cut-off value of 40.5.</p> Conclusion <p>Neutrophil CD64 is a valuable biomarker for the early diagnosis of neonatal sepsis, providing rapid results with minimal blood volume requirement. It may also have potential utility in identifying early or subclinical infection in high-risk neonates. Further large-scale studies are required to validate its prognostic role and relationship with specific bacterial isolates.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diagnostic utility of neutrophil CD64 for early detection of neonatal sepsis in southwestern Nigeria: a comparative case–control study

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月8日

查看全文
  • Ibraheem A. Ajetunmobi,
  • Ann A. Ogbenna,
  • Bukola A. Sayomi,
  • Oyejide A. Olaniyan,
  • Kafayat A. Olowookere,
  • Musa A. Muhibi

摘要

Background

Neonatal sepsis remains a major global health challenge due to its contribution to infant morbidity and mortality. Early diagnosis is often difficult because the clinical presentation is nonspecific. Despite extensive research, no biomarker has yet achieved the required sensitivity, specificity and minimal blood volume to avoid hospital-acquired anaemia in newborns. Identifying a reliable early marker could improve therapeutic decision making and reduce unnecessary antibiotics use in neonates with suspected infection.

Methodology

We conducted a hospital-based comparative case-control study in the special care baby units of two tertiary hospitals in Oyo state, Nigeria, between November 2018 and March 2019. A total of 120 neonates, aged 0–72 h were enrolled: 65 with suspected sepsis and 55 apparently healthy controls. Neutrophil CD64 expression was assessed using flow cytometry, Full Blood Count with differential and blood culture were also evaluated for every participant. Data were analyzed with SPSS Version 21 and statistical significance was set at P < 0.05.

Results

Neonates with suspected sepsis demonstrated significantly higher white blood cell counts and band neutrophil percentages than apparently healthy controls. The neutrophil CD64 expression was significantly higher in the sepsis group. Receiver operating characteristic analysis showed that neutrophil CD64 had an area under the curve (AUC) of 0.894 with sensitivity of 93.8% and specificity of 88.1% at cut-off value of 125.53. Percentage neutrophil CD64 demonstrated an AUC of 0.887 with a sensitivity of 93.8% and specificity of 88.1% at cut-off value of 40.5.

Conclusion

Neutrophil CD64 is a valuable biomarker for the early diagnosis of neonatal sepsis, providing rapid results with minimal blood volume requirement. It may also have potential utility in identifying early or subclinical infection in high-risk neonates. Further large-scale studies are required to validate its prognostic role and relationship with specific bacterial isolates.