Background <p>This study aimed to evaluate the impact of sexual health training on sexual myths, sexual function, and the quality of sexual life among pregnant women.</p> Methods <p>The study employed a pretest-posttest design. Participants were randomly assigned to either the intervention group (<i>n</i> = 45) or the control group (<i>n</i> = 45). Both groups completed a sociodemographic data form, a questionnaire on myths about sexual activity during pregnancy, the Female Sexual Function Index (FSFI), and the Sexual Quality of Life-Female (SQOL-F) questionnaire.</p> Results <p>The mean age of the participants was 27.58 ± 5.36 years, and the mean gestational age was 35.14 ± 5.52 weeks. After receiving sexual health training, women in the intervention group showed an increase in their SQOL-F scores and a decrease in their belief in sexual myths (<i>p</i> &lt; 0.05). No significant differences were observed between the groups regarding educational level, desire for the current pregnancy, and the frequency of sexual intercourse before and during pregnancy (<i>p</i> &gt; 0.05).</p> Conclusions <p>The sexual function of the participants was found to be inadequate. However, after receiving sexual health/reproductive health training, their sexual quality of life and sexual function scores increased. Furthermore, their attitudes towards sexual myths significantly changed in a positive direction. These findings indicate the importance of sexual health/reproductive health training during pregnancy.</p> Trial registration <p>Clinical Trials NCT06735677. Date of registration 11.12.2024 (Retrospectively registered).</p>