Purpose <p>Grounded on the Technology Acceptance Model and integrating theories of perceived risk and trust, this study explores the willingness of adults receiving hemodialysis to use mobile healthcare application services as well as its influencing factors.</p> Individuals and methods <p>Using convenience sampling, 313 adults receiving hemodialysis treated in a comprehensive hospital in Yangzhou City were selected as survey participants from January to March 2024. The study utilized a general information questionnaire and a specific questionnaire assessing the willingness of individuals on hemodialysis to use mobile healthcare application services. A structural equation model was constructed to analyze the influencing factors.</p> Results <p>The scores for each dimension assessing the willingness of adults receiving hemodialysis to use mobile healthcare application services questionnaire were as follows: Perceived Risk (3.31 ± 1.20), Perceived Trust (3.63 ± 0.85), Social Influence (3.84 ± 0.86), Perceived Ease of Use (3.57 ± 0.96), Perceived Usefulness (3.69 ± 0.84), Individual Innovativeness (4.02 ± 0.97), and Willingness to Use (3.85 ± 0.92). Structural equation model analysis indicated that Perceived Ease of Use, Perceived Usefulness, and Individual Innovativeness have a positive impact on the Willingness to Use, with effect values of 0.25, 0.38, and 0.21, respectively. There differences were statistically significant (all <i>P</i> &lt; 0.05).</p> Conclusion <p>The willingness level of adults receiving hemodialysis to use mobile healthcare application services is above average. Key factors influencing the willingness of individuals on hemodialysis to use these services are Perceived Ease of Use, Perceived Usefulness, and Individual Innovativeness.</p> Clinical trial number <p>ChiCTR2400087782.</p>