Background <p>Many kidney transplant recipients continue to be affected by multiple symptoms even after their transplant. Mindfulness may be helpful to alleviate some of the symptoms.</p> Objectives <p>Our current study aimed to determine the efficacy of a single session of 20-minute mindful breathing in alleviating multiple symptoms in kidney transplant patients.</p> Methods <p>Adult kidney transplant patients with at least one symptom scoring ≥ 4/10 based on the Edmonton Symptom Assessment Scale (ESAS) were recruited from November 2020 to May 2021. Participants were randomly assigned to either 20-minute mindful breathing or control.</p> Results <p>Sixty participants were recruited and randomly assigned to intervention (<i>N</i> = 30) or control (<i>N</i> = 30). There was statistically significant greater reduction of the total ESAS score in the intervention group compared with the control group (mean difference = -4.833, confidence interval = -7.837, -1.830, t = -3.251, <i>p</i> = 0002).</p> Conclusion <p>Our results provided evidence that 20-minute mindful breathing could reduce multiple symptoms rapidly in kidney transplant recipients.</p>