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<p>Carbapenem-Resistant <i>Acinetobacter baumannii</i> (CRAB) poses a major public health threat due to its multidrug resistance and high mortality rates, necessitating rapid and accurate diagnostic tools for timely intervention and infection control. This study developed and evaluated a novel detection method combining duplex recombinase polymerase amplification (DRPA) with a lateral flow strip (LFS) for the rapid visual identification of CRAB. The DRPA-LFS assay targets <i>bla</i><sub>OXA−23</sub> and <i>bla</i><sub>OXA−51</sub> genes, enabling isothermal amplification at 37&#xa0;°C followed by a simple visual readout. The method demonstrated high sensitivity (10<sup>2</sup> CFU). Results can be obtained within 40&#xa0;min without the need for complex instrumentation, making it suitable for resource-limited environments. the method offers a portable, user-friendly, and cost-effective solution for CRAB detection.</p>

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Development of a novel duplex recombinase polymerase amplification (DRPA) assay for rapid detection of carbapenem-resistant Acinetobacter baumannii

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月24日

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  • Chenyan Yuan,
  • Ting Zhang,
  • Juan Huo,
  • Wenxue Liang,
  • Lei Wang,
  • Kun Wang

摘要

Carbapenem-Resistant Acinetobacter baumannii (CRAB) poses a major public health threat due to its multidrug resistance and high mortality rates, necessitating rapid and accurate diagnostic tools for timely intervention and infection control. This study developed and evaluated a novel detection method combining duplex recombinase polymerase amplification (DRPA) with a lateral flow strip (LFS) for the rapid visual identification of CRAB. The DRPA-LFS assay targets blaOXA−23 and blaOXA−51 genes, enabling isothermal amplification at 37 °C followed by a simple visual readout. The method demonstrated high sensitivity (102 CFU). Results can be obtained within 40 min without the need for complex instrumentation, making it suitable for resource-limited environments. the method offers a portable, user-friendly, and cost-effective solution for CRAB detection.