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Background <p>Tuberculosis (TB) remains highly prevalent in many countries and is a significant contributor to infectious disease mortality. Combating TB requires monitoring the global circulation of strains, and analyzing the prevalence of specific strains within populations. Currently, whole-genome sequencing (WGS) enables the precise tracking of TB transmission. Here, we utilized spoligotyping combined with whole-genome sequencing (WGS) to identify cases of <i>M. tuberculosis</i> transmission in Poland. Our study aimed to identify <i>M. tuberculosis</i> clones transmitting in Poland before the beginning of the war in Ukraine and the influx of refugees.</p> Methods <p>We used spoligotyping data to identify <i>M. tuberculosis</i> isolates that were possibly transmitted. We used WGS to confirm transmission, and to identify transmission chains.</p> Results <p>A total of 163 isolates were successfully sequenced with high-quality genomic data. Two significant clusters were identified: a pan-susceptible Euro-American lineage cluster (WGS-Cluster 3, <i>n</i> = 32, 19.6%) associated with patients experiencing homelessness and centered in Bydgoszcz (2006–2014); and a multidrug-resistant Beijing lineage cluster (WGS-Cluster 13, <i>n</i> = 32, 19.6%) emerging between 2016 and 2020 across five cities.</p> Conclusion <p><i>M. tuberculosis</i> transmission should be monitored with routine WGS for better TB control in Poland.</p>

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Nationwide whole-genome sequencing reveals local transmission of Mycobacterium tuberculosis in Poland, 2003–2020

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月25日

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  • Alina Minias,
  • Lidia Fiedorowicz,
  • Monika Kozińska,
  • Anna Zabost,
  • Jakub Lach,
  • Marcin Słomka,
  • Dominik Strapagiel,
  • Jarosław Dziadek,
  • Ewa Augustynowicz-Kopeć

摘要

Background

Tuberculosis (TB) remains highly prevalent in many countries and is a significant contributor to infectious disease mortality. Combating TB requires monitoring the global circulation of strains, and analyzing the prevalence of specific strains within populations. Currently, whole-genome sequencing (WGS) enables the precise tracking of TB transmission. Here, we utilized spoligotyping combined with whole-genome sequencing (WGS) to identify cases of M. tuberculosis transmission in Poland. Our study aimed to identify M. tuberculosis clones transmitting in Poland before the beginning of the war in Ukraine and the influx of refugees.

Methods

We used spoligotyping data to identify M. tuberculosis isolates that were possibly transmitted. We used WGS to confirm transmission, and to identify transmission chains.

Results

A total of 163 isolates were successfully sequenced with high-quality genomic data. Two significant clusters were identified: a pan-susceptible Euro-American lineage cluster (WGS-Cluster 3, n = 32, 19.6%) associated with patients experiencing homelessness and centered in Bydgoszcz (2006–2014); and a multidrug-resistant Beijing lineage cluster (WGS-Cluster 13, n = 32, 19.6%) emerging between 2016 and 2020 across five cities.

Conclusion

M. tuberculosis transmission should be monitored with routine WGS for better TB control in Poland.