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Background <p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a major pathogen causing ear infections. However, genomic epidemiology and determinants influencing transmission of ear infections associated MRSA (EIA-MRSA) in community remain unknown.</p> Methods <p>In 2020–2021, 105 EIA-MRSA isolates were collected and sequenced from outpatients across different households in Shanghai, China. Antimicrobial susceptibility testing, core genome MLST, and phylodynamic analyses were conducted to characterize EIA-MRSA dissemination.</p> Results <p>Quinolone resistance was identified as a risk factor for EIA-MRSA spread (OR 9, [95% CI 3–31]). The ST764 clone and two subclones of ST22-PT hypervirulent clone have developed an extensive quinolone-resistant (eQR) phenotype, conferring additional resistance to advanced quinolones due to the accumulation of four mutations in <i>gyrA</i> (S84L and either S85P, E88K, or E88G) and <i>parC</i> (S80F and either E84K or E84G). These ST764- and ST22-PT-eQR isolates were highly transmissible and showed increased resistance to other commonly used antimicrobials, posing potential high-risk clones. The eQR phenotype may be inherent to the ST764 lineage, which emerged in the late 1980s, coinciding with the widespread fluoroquinolone usage. The ST22-PT-eQR subclones emerged in around 2017 and are accumulating resistance genes.</p> Conclusion <p>Vigilance is crucial for eQR high-risk clones, particularly the convergent ST22-PT-eQR subclones that accumulate resistance and virulence traits, posing risks for ear infections.</p> Clinical trial number <p>Not applicable.</p>

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Genomic insights into the spread of methicillin-resistant Staphylococcus aureus involved in ear infections

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月6日

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  • Zhewei Sun,
  • Jinhong Chen,
  • Chunhong Liu,
  • Yueru Tian,
  • Fuqi Ai,
  • Jiaying Du,
  • Wangxiao Zhou,
  • Wenjun Cao,
  • Ming Guan,
  • Baixing Ding

摘要

Background

Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen causing ear infections. However, genomic epidemiology and determinants influencing transmission of ear infections associated MRSA (EIA-MRSA) in community remain unknown.

Methods

In 2020–2021, 105 EIA-MRSA isolates were collected and sequenced from outpatients across different households in Shanghai, China. Antimicrobial susceptibility testing, core genome MLST, and phylodynamic analyses were conducted to characterize EIA-MRSA dissemination.

Results

Quinolone resistance was identified as a risk factor for EIA-MRSA spread (OR 9, [95% CI 3–31]). The ST764 clone and two subclones of ST22-PT hypervirulent clone have developed an extensive quinolone-resistant (eQR) phenotype, conferring additional resistance to advanced quinolones due to the accumulation of four mutations in gyrA (S84L and either S85P, E88K, or E88G) and parC (S80F and either E84K or E84G). These ST764- and ST22-PT-eQR isolates were highly transmissible and showed increased resistance to other commonly used antimicrobials, posing potential high-risk clones. The eQR phenotype may be inherent to the ST764 lineage, which emerged in the late 1980s, coinciding with the widespread fluoroquinolone usage. The ST22-PT-eQR subclones emerged in around 2017 and are accumulating resistance genes.

Conclusion

Vigilance is crucial for eQR high-risk clones, particularly the convergent ST22-PT-eQR subclones that accumulate resistance and virulence traits, posing risks for ear infections.

Clinical trial number

Not applicable.