Scholarlink

<p>The primary goal of antiretroviral therapy (ART) is to suppress viral replication to undetectable levels (&lt; 50 copies/mL). Despite achieving complete viral suppression, 10–40% of individuals on ART do not adequately restore their CD4 + T-cell count, being defined as immunological non-responders (INR). Factors such as sex, age at treatment initiation, coinfections, and pre-ART CD4 + T-cell count may influence this insufficient recovery. This impairment can also result from poor production or exacerbated destruction of CD4 + T-cells, particularly through extrinsic pathway-mediated apoptosis involving Fas/FasL and caspase-3. Thus, this study aimed to evaluate the expression profile of extrinsic apoptosis pathway genes (<i>CASP3</i>, <i>FAS</i>, <i>FASLG</i>) in adult male HIV patients on ART. The patients were stratified as immunological responders (<i>n</i> = 25) and immunological non-responders (<i>n</i> = 8) based on the increase and total count of CD4 + T-cells. Significant differences for <i>CASP3</i> (FC = 1.39, <i>p</i> = 0.047) and <i>FASLG</i> (FC = 1.94, <i>p</i> &lt; 0.0001) gene expressions were identified between IR and INR groups, but not for <i>FAS</i> (FC=-1.2, <i>p</i> = 0.638). This study indicates increased apoptotic pathway gene expression in INR and highlights the influence of cell destruction mechanisms on immunological recovery.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The influence of extrinsic apoptosis gene expression on immunological reconstitution of male ART-treated PLHIV

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月18日

查看全文
  • Henrique Fernando Lopes-Araujo,
  • Maria Carolina Santos Guedes,
  • Luiz Cláudio Arraes De Alencar,
  • Wlisses Henrique Veloso Carvalho-Silva,
  • Lílian Maria Lapa Montenegro,
  • Rafael Lima Guimarães

摘要

The primary goal of antiretroviral therapy (ART) is to suppress viral replication to undetectable levels (< 50 copies/mL). Despite achieving complete viral suppression, 10–40% of individuals on ART do not adequately restore their CD4 + T-cell count, being defined as immunological non-responders (INR). Factors such as sex, age at treatment initiation, coinfections, and pre-ART CD4 + T-cell count may influence this insufficient recovery. This impairment can also result from poor production or exacerbated destruction of CD4 + T-cells, particularly through extrinsic pathway-mediated apoptosis involving Fas/FasL and caspase-3. Thus, this study aimed to evaluate the expression profile of extrinsic apoptosis pathway genes (CASP3, FAS, FASLG) in adult male HIV patients on ART. The patients were stratified as immunological responders (n = 25) and immunological non-responders (n = 8) based on the increase and total count of CD4 + T-cells. Significant differences for CASP3 (FC = 1.39, p = 0.047) and FASLG (FC = 1.94, p < 0.0001) gene expressions were identified between IR and INR groups, but not for FAS (FC=-1.2, p = 0.638). This study indicates increased apoptotic pathway gene expression in INR and highlights the influence of cell destruction mechanisms on immunological recovery.