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Background <p>This study investigated whether fecal microbiota transfer (FMT) from Turkish celiac disease (CD) patients is associated with the induction of celiac-like enteropathy features&#xa0;in a rat model.</p> Methods <p>Wistar rats received FMT from 10 CD patients or 10 healthy controls following microbiota depletion. Physiological, histopathological (Marsh-like classification), and inflammatory markers were evaluated.</p> Results <p>The celiac disease fecal microbiota recipient rats (CD-FMT rats)group exhibited weight loss (<i>p</i> &lt; 0.0001) and&#xa0;celiac-like histopathological features in 90% of cases. These included marked villous atrophy and intraepithelial lymphocyte counts &gt; 20 per high-power field (HPF). A weighted kappa analysis (0.667) demonstrated a moderate association between donor Marsh scores and recipient histopathology. Systemic inflammation in the CD-FMT rats group was marked by a two-fold increase in serum IL-17 (~ 245&#xa0;pg/ml; <i>p</i> &lt; 0.0001) and a three-fold increase in IFN-γ (~ 95&#xa0;pg/ml; <i>p</i> &lt; 0.001). Additionally, mucosal mRNA expression of IL-15, IL-21, TNF-α, and IFN-α was upregulated approximately three-fold (<i>p</i> &lt; 0.001). Serum β-actin levels were significantly elevated (~ 9.5&#xa0;ng/ml; <i>p</i> &lt; 0.0001), suggesting increased intestinal injury in this experimental setting.</p> Conclusion <p>FMT from Turkish CD patients was associated with the induction of celiac-like enteropathy features in this rat model. These findings suggest that gut microbiota from celiac disease patients may contribute to celiac-like mucosal and immune alterations in this experimental model, although it is not sufficient alone to induce disease and should be interpreted as a modulatory rather than causal factor.</p> Graphical Abstract <p></p>

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FMT from Turkish patients with celiac disease is associated with celiac-like enteropathy features in a rat model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月8日

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  • Rugiyya Samadzade,
  • Salih Macin,
  • Babek Alibayov,
  • Zeynep Celik,
  • Mehmet Burak Ates,
  • Muslu Kazım Korez,
  • Huseyin Korkmaz,
  • Duygu Findik

摘要

Background

This study investigated whether fecal microbiota transfer (FMT) from Turkish celiac disease (CD) patients is associated with the induction of celiac-like enteropathy features in a rat model.

Methods

Wistar rats received FMT from 10 CD patients or 10 healthy controls following microbiota depletion. Physiological, histopathological (Marsh-like classification), and inflammatory markers were evaluated.

Results

The celiac disease fecal microbiota recipient rats (CD-FMT rats)group exhibited weight loss (p < 0.0001) and celiac-like histopathological features in 90% of cases. These included marked villous atrophy and intraepithelial lymphocyte counts > 20 per high-power field (HPF). A weighted kappa analysis (0.667) demonstrated a moderate association between donor Marsh scores and recipient histopathology. Systemic inflammation in the CD-FMT rats group was marked by a two-fold increase in serum IL-17 (~ 245 pg/ml; p < 0.0001) and a three-fold increase in IFN-γ (~ 95 pg/ml; p < 0.001). Additionally, mucosal mRNA expression of IL-15, IL-21, TNF-α, and IFN-α was upregulated approximately three-fold (p < 0.001). Serum β-actin levels were significantly elevated (~ 9.5 ng/ml; p < 0.0001), suggesting increased intestinal injury in this experimental setting.

Conclusion

FMT from Turkish CD patients was associated with the induction of celiac-like enteropathy features in this rat model. These findings suggest that gut microbiota from celiac disease patients may contribute to celiac-like mucosal and immune alterations in this experimental model, although it is not sufficient alone to induce disease and should be interpreted as a modulatory rather than causal factor.

Graphical Abstract