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<p>Sepsis is a systemic inflammatory response syndrome triggered by infection, which can lead to multiple organ dysfunction. This study untangles the synergistic multi-mechanistic effects of the natural flavonoid apigenin in ameliorating this pathological process. Utilizing a murine sepsis model and Caco-2 cell line, we systematically investigated the impact of apigenin on intestinal barrier function. Apigenin treatment (50&#xa0;mg/kg) markedly improved intestinal barrier integrity, as shown by reduced serum FITC-dextran levels and restored expression of tight junction proteins Occludin, Claudin-1, and ZO-1. The compound simultaneously attenuated systemic inflammation by lowering IL-6 and TNF-α levels. Network pharmacology and molecular docking identified AKT1 and MMP-9 as key molecular targets of apigenin, which was experimentally validated through observed suppression of MMP-9 and COX-2 protein expression. These results demonstrate apigenin’s capacity to preserve intestinal barrier function during sepsis through coordinated anti-inflammatory and barrier-repair mechanisms.</p>

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Apigenin mitigates intestinal barrier dysfunction in sepsis by modulating the AKT signaling pathway

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月30日

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  • Zheng Lijun,
  • Wang Liping,
  • Zhao Dandan,
  • Zhao Wenjie,
  • Xu Renjie,
  • Ji Xueyan,
  • Che Wenqing,
  • Li Chen,
  • Zhao Lu

摘要

Sepsis is a systemic inflammatory response syndrome triggered by infection, which can lead to multiple organ dysfunction. This study untangles the synergistic multi-mechanistic effects of the natural flavonoid apigenin in ameliorating this pathological process. Utilizing a murine sepsis model and Caco-2 cell line, we systematically investigated the impact of apigenin on intestinal barrier function. Apigenin treatment (50 mg/kg) markedly improved intestinal barrier integrity, as shown by reduced serum FITC-dextran levels and restored expression of tight junction proteins Occludin, Claudin-1, and ZO-1. The compound simultaneously attenuated systemic inflammation by lowering IL-6 and TNF-α levels. Network pharmacology and molecular docking identified AKT1 and MMP-9 as key molecular targets of apigenin, which was experimentally validated through observed suppression of MMP-9 and COX-2 protein expression. These results demonstrate apigenin’s capacity to preserve intestinal barrier function during sepsis through coordinated anti-inflammatory and barrier-repair mechanisms.