Background <p>Amid growing demands, workforce shortages, and rising costs, the evolving role of diagnostics—driven by technological innovation—underscores the urgent need to rethink the diagnostic process with stakeholders at the center. Knowledge on their perspectives on innovation of the diagnostic process is lacking, while it could ensure alignment with their needs and expectations.</p> Aim <p>To identify perspectives on innovation of the diagnostic process in general practice care.</p> Design and setting <p>Q-methodological study with stakeholders with different profiles but an informed opinion on the topic.</p> Method <p>Participants individually sorted 57 statements based on what they thought is most important for innovation of the diagnostic process in general practice care. Statements were collected from literature, media, and group meetings. Factor analyses identified different perspectives, which were subsequently holistically interpreted.</p> Results <p>We identified five perspectives: 1-innovation through diagnostic transformation, 2-innovation in communication, 3-innovation from a doctor-centered perspective, 4-system reform before innovation: fixing the foundation first, and 5-ambivalence towards innovation. The perspectives differed mostly in their urge for system change and the role for technology in innovation. In some perspectives, changes in the current or future diagnostic system were identified, whereas in others not. Also, the degree to which technology was deemed as the promising way forward differed between the perspectives.</p> Conclusion <p>This study gives insight into prevailing perspectives of stakeholders on innovation of the diagnostic process in general practice care. Each perspective offers valuable insights, as all are essential for successful innovation of the diagnostic process.</p>