Scholarlink

Background <p><i>Lilium amoenum</i> E. H. Wilson ex Sealy is a wild lily species endemic to Yunnan, China, and is of considerable ornamental and scientific interest owing to its solitary, pendulous, fragrant, rose-purple flowers. Although the chloroplast genome of this species has been reported, its mitogenome has not yet been characterized. To address this gap, we employed a hybrid sequencing strategy combining Illumina short-read and Nanopore long-read data to assemble and annotate, for the first time, the complete mitogenome of <i>L. amoenum</i>.</p> Result <p>The mitogenome of <i>L. amoenum</i> exhibits a distinctive multichromosomal architecture comprising 13 circular chromosomes and one linear chromosome, with a total length of 789,335&#xa0;bp and an overall GC content of 45.11%. In total, 55 genes were annotated, including 37 protein-coding genes (PCGs), 3 rRNA genes, and 15 tRNA genes. We also identified 171 simple sequence repeats (SSRs), eight tandem repeats, and 509 dispersed repeats. Homology analysis detected 14 plastid-derived fragments totaling 4,723&#xa0;bp, representing 0.60% of the mitogenome. Collinearity analysis revealed extensive genomic rearrangements between <i>L. amoenum</i> and its close relatives, indicating substantial structural divergence during mitogenome evolution. Phylogenetic analysis based on the mitogenomes of 26 species showed that <i>L. amoenum</i> clusters with other members of <i>Lilium</i>, consistent with the APG IV classification system. In addition, 493&#xa0;C-to-U RNA editing sites in the coding regions of mitochondrial PCGs were predicted. Lastly, we successfully validated five randomly selected RNA editing sites.</p> Conclusion <p>This study provides the first complete mitogenome of <i>L. amoenum</i>, revealing its unique multi-chromosomal configuration. Moreover, it offers a valuable genomic resource for breeding, species identification, germplasm conservation, and phylogenetic studies within <i>Lilium</i> species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Complete mitogenome assembly of Lilium amoenum E. H. Wilson ex Sealy revealed its multiple chromosomal configurations, genome evolution, phylogenetic implications, and RNA-editing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月5日

查看全文
  • Yanbing Guo,
  • Ziwei Li,
  • Shengyue Yi,
  • Shuxian Li,
  • Wenyi Dong,
  • Hongzhi Wu

摘要

Background

Lilium amoenum E. H. Wilson ex Sealy is a wild lily species endemic to Yunnan, China, and is of considerable ornamental and scientific interest owing to its solitary, pendulous, fragrant, rose-purple flowers. Although the chloroplast genome of this species has been reported, its mitogenome has not yet been characterized. To address this gap, we employed a hybrid sequencing strategy combining Illumina short-read and Nanopore long-read data to assemble and annotate, for the first time, the complete mitogenome of L. amoenum.

Result

The mitogenome of L. amoenum exhibits a distinctive multichromosomal architecture comprising 13 circular chromosomes and one linear chromosome, with a total length of 789,335 bp and an overall GC content of 45.11%. In total, 55 genes were annotated, including 37 protein-coding genes (PCGs), 3 rRNA genes, and 15 tRNA genes. We also identified 171 simple sequence repeats (SSRs), eight tandem repeats, and 509 dispersed repeats. Homology analysis detected 14 plastid-derived fragments totaling 4,723 bp, representing 0.60% of the mitogenome. Collinearity analysis revealed extensive genomic rearrangements between L. amoenum and its close relatives, indicating substantial structural divergence during mitogenome evolution. Phylogenetic analysis based on the mitogenomes of 26 species showed that L. amoenum clusters with other members of Lilium, consistent with the APG IV classification system. In addition, 493 C-to-U RNA editing sites in the coding regions of mitochondrial PCGs were predicted. Lastly, we successfully validated five randomly selected RNA editing sites.

Conclusion

This study provides the first complete mitogenome of L. amoenum, revealing its unique multi-chromosomal configuration. Moreover, it offers a valuable genomic resource for breeding, species identification, germplasm conservation, and phylogenetic studies within Lilium species.