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<p><i>Prunus sibirica</i> L., an economically and ecologically significant Asian endemic species, faces severe pathogenic threats, yet its disease resistance mechanisms remain poorly understood. Using RNA-seq of pathogen-infected leaves, we identified PsWRKY75 as a potential resistance gene. Heterologous overexpression in poplars showed that PsWRKY75 enhanced disease resistance, promoting lignin and H₂O₂ accumulation in leaves. Through RNA-seq and promoter analysis, PsWRKY75 was identified as an upstream regulator of the H₂O₂-producing gene <i>PsRbohB</i> and lignin biosynthesis gene <i>PsLFP1</i>, validated by yeast one-hybrid, EMSA, and dual luciferase assays. Pathogen infection upregulates PsWRKY75, which directly activates <i>PsRbohB</i> and <i>PsLFP1</i> to enhance lignin/H₂O₂ accumulation, thereby strengthening disease resistance. This study reveals PsWRKY75 as a novel integrator of H₂O₂ signaling and lignin biosynthesis, providing genetic resources for woody plant disease resistance improvement via physiological and biochemical engineering.</p>

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PsWRKY75 orchestrates lignin biosynthesis and H₂O₂ metabolism to modulate disease resistance: a physiological and biochemical mechanism in Prunus sibirica L.

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月7日

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  • Qiuxian Bai,
  • Haiyan Lan,
  • Dan Liu

摘要

Prunus sibirica L., an economically and ecologically significant Asian endemic species, faces severe pathogenic threats, yet its disease resistance mechanisms remain poorly understood. Using RNA-seq of pathogen-infected leaves, we identified PsWRKY75 as a potential resistance gene. Heterologous overexpression in poplars showed that PsWRKY75 enhanced disease resistance, promoting lignin and H₂O₂ accumulation in leaves. Through RNA-seq and promoter analysis, PsWRKY75 was identified as an upstream regulator of the H₂O₂-producing gene PsRbohB and lignin biosynthesis gene PsLFP1, validated by yeast one-hybrid, EMSA, and dual luciferase assays. Pathogen infection upregulates PsWRKY75, which directly activates PsRbohB and PsLFP1 to enhance lignin/H₂O₂ accumulation, thereby strengthening disease resistance. This study reveals PsWRKY75 as a novel integrator of H₂O₂ signaling and lignin biosynthesis, providing genetic resources for woody plant disease resistance improvement via physiological and biochemical engineering.