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Background <p>ASR genes are a class of genes that are expressed in plants in response to abscisic acid, abiotic stress, and fruit ripening. While they have been successfully cloned and functionally characterized in various plant species, research on this gene in <i>Tamarix hispida</i> remains limited.</p> Results <p>In this study, six <i>ThASR</i> genes were cloned from <i>Tamarix hispida</i>. Expression analysis revealed that <i>ThASR</i> genes respond to salt stress. Specifically, <i>ThASR4</i> was found to be highly induced in both shoots and roots of <i>Tamarix hispida</i> under salt stress, thus prompting its selection for further functional characterization in salt stress responses. <i>ThASR4</i> is targeted to the nucleus and possesses transcriptional activity. Under salt stress conditions, compared with the wild-type (WT) lines, <i>ThASR4</i>-overexpressing <i>Arabidopsis thaliana</i> exhibited enhanced germination rate, longer primary roots, and higher fresh weight, indicating a marked improvement in salt tolerance. Correspondingly, both <i>ThASR4</i>-overexpressing <i>T. hispida</i> and <i>A. thaliana</i> plants exhibited significantly diminished levels of reactive oxygen species (ROS), malondialdehyde (MDA), and electrolyte leakage, along with elevated activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), as well as increased proline (Pro) content, compared to Control plants. In contrast, <i>ThASR4</i> RNA interference (RNAi) transgenic <i>T. hispida</i> displayed the opposite phenotypic and physiological outcomes. Furthermore, <i>ThASR4</i> was found to upregulate the expression of ROS scavenger-associated genes (<i>ThPOD1</i>,<i> ThSOD1</i> and <i>ThCAT3</i>) and salt Stress-Responsive genes (<i>ThSOS3</i>, <i>ThPIP2;5</i>, and <i>ThDREB</i>).</p> Conclusions <p><i>ThASR4</i> enhances salt tolerance in <i>T. hispida</i> and <i>A. thaliana</i> by improving ROS scavenging capacity. The present study provides a theoretical foundation for further investigation into the regulatory mechanism of <i>ThASR4</i> in the salt stress adaptation of <i>T. hispida</i>.</p>

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ThASR4 enhances salt tolerance in transgenic Tamarix and Arabidopsis by scavenging reactive oxygen species

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月15日

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  • Zhenyu Zhu,
  • Jie Wang,
  • Hanyang Liu,
  • Yu Zhang,
  • Xin Zhao,
  • Chao Wang

摘要

Background

ASR genes are a class of genes that are expressed in plants in response to abscisic acid, abiotic stress, and fruit ripening. While they have been successfully cloned and functionally characterized in various plant species, research on this gene in Tamarix hispida remains limited.

Results

In this study, six ThASR genes were cloned from Tamarix hispida. Expression analysis revealed that ThASR genes respond to salt stress. Specifically, ThASR4 was found to be highly induced in both shoots and roots of Tamarix hispida under salt stress, thus prompting its selection for further functional characterization in salt stress responses. ThASR4 is targeted to the nucleus and possesses transcriptional activity. Under salt stress conditions, compared with the wild-type (WT) lines, ThASR4-overexpressing Arabidopsis thaliana exhibited enhanced germination rate, longer primary roots, and higher fresh weight, indicating a marked improvement in salt tolerance. Correspondingly, both ThASR4-overexpressing T. hispida and A. thaliana plants exhibited significantly diminished levels of reactive oxygen species (ROS), malondialdehyde (MDA), and electrolyte leakage, along with elevated activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), as well as increased proline (Pro) content, compared to Control plants. In contrast, ThASR4 RNA interference (RNAi) transgenic T. hispida displayed the opposite phenotypic and physiological outcomes. Furthermore, ThASR4 was found to upregulate the expression of ROS scavenger-associated genes (ThPOD1, ThSOD1 and ThCAT3) and salt Stress-Responsive genes (ThSOS3, ThPIP2;5, and ThDREB).

Conclusions

ThASR4 enhances salt tolerance in T. hispida and A. thaliana by improving ROS scavenging capacity. The present study provides a theoretical foundation for further investigation into the regulatory mechanism of ThASR4 in the salt stress adaptation of T. hispida.