Scholarlink

Background <p>Wheat grain development is the core biological process that determines the formation of grain yield and quality. However, as a heterohexaploid crop, wheat has not been systematically analyzed for its molecular regulatory network governing grain development.</p> Methods <p>In this study, the wheat cultivar 'Dianmai 24' was used as the experimental material to identify the phenotypic characteristics of grains at three key grain filling stages, namely 7&#xa0;days (d), 14 d, and 21 d after anthesis, and to integrate transcriptomic, non-targeted metabolomic, and proteomic analyses.</p> Results <p>Phenotypic analysis showed that there were significant differences in grain width and biomass among the three stages, which exhibited the typical growth characteristics during the grain filling period. A total of 2665 metabolites were identified in the metabolomic analysis, among which flavonoids, amino acids and their derivatives, and lipid metabolites were the main differential metabolic components across different developmental stages. Transcriptomic analysis yielded a total of 85.13&#xa0;Gb of clean data. A total of 10,445, 16,306, and 8,437 differentially expressed genes (DEGs) were screened out in the three comparison groups, which were significantly enriched in metabolic pathways, photosynthesis-antenna proteins, biosynthesis of secondary metabolites, starch and sucrose metabolism, and carbon metabolism. A total of 17,729 proteins were identified by proteomic analysis, among which α-amylase/trypsin inhibitor CM3, β-amylase, and globulin-3A accumulated continuously across the three stages, and these proteins may play a core role in the process of grain development.</p> Conclusions <p>Through multi-omics analysis, the α-linolenic acid metabolic pathway was identified as the core regulatory pathway, and 15 key genes, 17 core proteins, and 11 characteristic metabolites were screened to participate in the molecular regulatory network of grain filling. This study systematically analyzed the molecular regulatory mechanism of the wheat grain filling stage and provided important candidate genes and metabolic targets for the breeding of high-yield and high-quality wheat.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrative multi-omics analysis uncovers the regulatory network of wheat grain development during grain filling

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月26日

查看全文
  • Xuqin Wang,
  • Chen Chen,
  • Yuanyuan Zuo,
  • Guofei Jiang,
  • Hanxue Li,
  • Yuming Xie,
  • Jianlong Zhou,
  • Huanyao Xia,
  • Chuanli Zhang,
  • Peng Qin

摘要

Background

Wheat grain development is the core biological process that determines the formation of grain yield and quality. However, as a heterohexaploid crop, wheat has not been systematically analyzed for its molecular regulatory network governing grain development.

Methods

In this study, the wheat cultivar 'Dianmai 24' was used as the experimental material to identify the phenotypic characteristics of grains at three key grain filling stages, namely 7 days (d), 14 d, and 21 d after anthesis, and to integrate transcriptomic, non-targeted metabolomic, and proteomic analyses.

Results

Phenotypic analysis showed that there were significant differences in grain width and biomass among the three stages, which exhibited the typical growth characteristics during the grain filling period. A total of 2665 metabolites were identified in the metabolomic analysis, among which flavonoids, amino acids and their derivatives, and lipid metabolites were the main differential metabolic components across different developmental stages. Transcriptomic analysis yielded a total of 85.13 Gb of clean data. A total of 10,445, 16,306, and 8,437 differentially expressed genes (DEGs) were screened out in the three comparison groups, which were significantly enriched in metabolic pathways, photosynthesis-antenna proteins, biosynthesis of secondary metabolites, starch and sucrose metabolism, and carbon metabolism. A total of 17,729 proteins were identified by proteomic analysis, among which α-amylase/trypsin inhibitor CM3, β-amylase, and globulin-3A accumulated continuously across the three stages, and these proteins may play a core role in the process of grain development.

Conclusions

Through multi-omics analysis, the α-linolenic acid metabolic pathway was identified as the core regulatory pathway, and 15 key genes, 17 core proteins, and 11 characteristic metabolites were screened to participate in the molecular regulatory network of grain filling. This study systematically analyzed the molecular regulatory mechanism of the wheat grain filling stage and provided important candidate genes and metabolic targets for the breeding of high-yield and high-quality wheat.