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Background <p>Embryo abortion is a major factor limiting hybrid breeding efficiency in jujube (<i>Ziziphus jujuba</i> Mill.). To investigate whether the ABCG transporter gene <i>ZjABCG15</i> is associated with reproductive development and ovule/seed abortion-related phenotypes, we performed bioinformatic analysis, expression profiling, and heterologous functional validation using the jujube cultivar ‘Zhongqiusucui’.</p> Results <p>The cDNA sequence of <i>ZjABCG15</i> is 2,019&#xa0;bp and encodes a 672-amino-acid protein. Bioinformatic analysis suggested that ZjABCG15 is a plasma membrane-associated ABCG transporter. Subcellular localization further confirmed its plasma membrane localization. Quantitative real-time PCR (qRT-PCR) showed that <i>ZjABCG15</i> was expressed in multiple jujube tissues, with relatively high transcript levels in leaves and kernels. For functional validation, overexpression and RNA interference (RNAi) constructs of <i>ZjABCG15</i> were introduced into Arabidopsis thaliana. Compared with the wild type, the average seed yield per plant increased by 44.2% in the overexpression line, but decreased by 12.65% in the RNAi line. In addition, RNAi plants showed shorter siliques, fewer seeds, and increased frequencies of seed abortion-related phenotypes, whereas overexpression plants produced more siliques and plump seeds.</p> Conclusions <p>These results suggest that <i>ZjABCG15</i> is a plasma membrane-localized ABCG transporter gene associated with ovule-to-seed development in jujube. Heterologous functional analysis in Arabidopsis supports its conserved positive role in reproductive development, whereas its direct role and regulatory mechanism in jujube still require further validation.</p>

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Bioinformatic analysis and functional characterization of ZjABCG15, a plasma membrane-localized ABCG transporter associated with reproductive development in Ziziphus jujuba Mill. ‘Zhongqiusucui’

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月7日

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  • Yutong Li,
  • Juan Chen,
  • Fengxia Shao,
  • Xinxin Lian,
  • Kuo Yang,
  • Sen Wang,
  • Yafeng Wen

摘要

Background

Embryo abortion is a major factor limiting hybrid breeding efficiency in jujube (Ziziphus jujuba Mill.). To investigate whether the ABCG transporter gene ZjABCG15 is associated with reproductive development and ovule/seed abortion-related phenotypes, we performed bioinformatic analysis, expression profiling, and heterologous functional validation using the jujube cultivar ‘Zhongqiusucui’.

Results

The cDNA sequence of ZjABCG15 is 2,019 bp and encodes a 672-amino-acid protein. Bioinformatic analysis suggested that ZjABCG15 is a plasma membrane-associated ABCG transporter. Subcellular localization further confirmed its plasma membrane localization. Quantitative real-time PCR (qRT-PCR) showed that ZjABCG15 was expressed in multiple jujube tissues, with relatively high transcript levels in leaves and kernels. For functional validation, overexpression and RNA interference (RNAi) constructs of ZjABCG15 were introduced into Arabidopsis thaliana. Compared with the wild type, the average seed yield per plant increased by 44.2% in the overexpression line, but decreased by 12.65% in the RNAi line. In addition, RNAi plants showed shorter siliques, fewer seeds, and increased frequencies of seed abortion-related phenotypes, whereas overexpression plants produced more siliques and plump seeds.

Conclusions

These results suggest that ZjABCG15 is a plasma membrane-localized ABCG transporter gene associated with ovule-to-seed development in jujube. Heterologous functional analysis in Arabidopsis supports its conserved positive role in reproductive development, whereas its direct role and regulatory mechanism in jujube still require further validation.