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<p><i>Perilla frutescens</i> (L.) is an important plant in traditional Chinese medicine and ornamental horticulture. However, classical genetic studies in this species remain limited. To perform QTL mapping for its important traits, the whole-genome resequencing of an F2 segregating population (<i>n</i> = 140) was conducted to construct a high-density genetic map. The sequencing generated 2,865.46 Gb of clean data. And the resulting high-density genetic map comprised 1,321.55&#xa0;cM across 20 linkage groups, consisting of 2,289 recombination bins that integrated 9,599,671 SNP markers. The map exhibited an average interval of 0.58&#xa0;cM per marker. To identify candidate genes associated with flowering traits, integrative QTL analysis delineated four consensus QTL regions associated with flower bud differentiation and initial flowering time. The 122 candidate genes were identified in these regions, including <i>PfBBX8</i>, a member of the CCT domain-containing family. Gene Ontology (GO) enrichment analysis suggested that <i>PfBBX8</i> may serve as a key regulator in photoperiod-mediated flowering pathways. Heterologous overexpression of <i>PfBBX8</i> in <i>Arabidopsis thaliana</i> significantly promoted flowering, whereas a natural C/T mutation in <i>PfBBX8</i> resulted in substantially delayed flowering relative to transgenic lines overexpressing the wild-type allele. Moreover, overexpression of <i>PfBBX8</i> upregulated downstream flowering integrators such as CO and FT, indicating its role in activating flowering pathways. These findings provide valuable high-density genetic map and flowering regulation for molecular breeding in <i>Perilla</i>.</p>

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Construction of high-density genetic map and QTL mapping of flowering traits in Perilla frutescens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月1日

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  • Xiuzai Zou,
  • Wenhui Zhou,
  • Minting Lu,
  • Guanwen Xie,
  • Duan Wu,
  • Gengyun Zhang,
  • Tong Wei,
  • Qi Shen

摘要

Perilla frutescens (L.) is an important plant in traditional Chinese medicine and ornamental horticulture. However, classical genetic studies in this species remain limited. To perform QTL mapping for its important traits, the whole-genome resequencing of an F2 segregating population (n = 140) was conducted to construct a high-density genetic map. The sequencing generated 2,865.46 Gb of clean data. And the resulting high-density genetic map comprised 1,321.55 cM across 20 linkage groups, consisting of 2,289 recombination bins that integrated 9,599,671 SNP markers. The map exhibited an average interval of 0.58 cM per marker. To identify candidate genes associated with flowering traits, integrative QTL analysis delineated four consensus QTL regions associated with flower bud differentiation and initial flowering time. The 122 candidate genes were identified in these regions, including PfBBX8, a member of the CCT domain-containing family. Gene Ontology (GO) enrichment analysis suggested that PfBBX8 may serve as a key regulator in photoperiod-mediated flowering pathways. Heterologous overexpression of PfBBX8 in Arabidopsis thaliana significantly promoted flowering, whereas a natural C/T mutation in PfBBX8 resulted in substantially delayed flowering relative to transgenic lines overexpressing the wild-type allele. Moreover, overexpression of PfBBX8 upregulated downstream flowering integrators such as CO and FT, indicating its role in activating flowering pathways. These findings provide valuable high-density genetic map and flowering regulation for molecular breeding in Perilla.