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<p>As an important turfgrass and forage grass, bermudagrass exhibits high tolerance to submergence stress, however, the molecular mechanisms are poorly clarified and required further elucidation. In this study, physiological analysis and transcriptome sequencing of bermudagrass under submergence stress and post-submergence recovery stage were conducted. Results showed that submergence exerted adverse effects on bermudagrass with decreased stolon length and photosynthetic pigment content. Many differentially expressed unigenes/genes (DEGs) were identified in shoots and roots under submergence and post-submergence stresses. Among these DEGs, 830 DEGs were annotated as transcription factors (TFs) and 104 TFs belong to APETALA 2/ethylene response element binding protein (AP2-EREBP) family. KEGG pathway enrichment analysis revealed that 42 and 4 (11 and 19) KEGG pathways were significantly enriched after submergence and post-submergence recovery in roots (shoots), including plant hormone signaling, sulfur metabolism, carbohydrate metabolism and phenylpropanoid biosynthesis. Moreover, protein-protein interaction (PPI) network was conducted, several hub genes were identified, including those encoding ribosomal proteins, translation initiation factor, persulfide dioxygenase ETHE1 and Enolase 1. Finally, a peroxidase gene <i>CdPER47</i> were selected and cloned, <i>CdPER47</i> exhibited significantly differential expression in roots under both submergence and post-submergence stresses. Heterogeneous overexpression of <i>CdPER47</i> in Arabidopsis increased its survival rate significantly after submergence stress. Based on the above results, a hypothetical model was proposed for bermudagrass responses to submergence and post-submergence stresses. Our study reveals that bermudagrass responds to submergence and post-submergence stresses through extensive transcriptional reprogramming involving AP2-EREBP transcription factors, multiple metabolic pathways, and key hub genes including the functionally validated peroxidase gene <i>CdPER47</i>. Together, this study will provide valuable references for developing submergence-tolerance germplasm in the future.</p>

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Transcriptome analysis reveals the mechanism and key role of CdPER47 in bermudagrass (Cynodon dactylon) adaptation to submergence and post-submergence stresses

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月7日

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  • Weitao Jia,
  • Sihan Hou,
  • Songyi Yang,
  • Yuhan Zou,
  • Wenqing Dou,
  • Kai Zhu,
  • Cunfeng Zhao,
  • Shihui Zou,
  • Huanhuan Liu,
  • Lijiao Ai,
  • Shengjun Wu

摘要

As an important turfgrass and forage grass, bermudagrass exhibits high tolerance to submergence stress, however, the molecular mechanisms are poorly clarified and required further elucidation. In this study, physiological analysis and transcriptome sequencing of bermudagrass under submergence stress and post-submergence recovery stage were conducted. Results showed that submergence exerted adverse effects on bermudagrass with decreased stolon length and photosynthetic pigment content. Many differentially expressed unigenes/genes (DEGs) were identified in shoots and roots under submergence and post-submergence stresses. Among these DEGs, 830 DEGs were annotated as transcription factors (TFs) and 104 TFs belong to APETALA 2/ethylene response element binding protein (AP2-EREBP) family. KEGG pathway enrichment analysis revealed that 42 and 4 (11 and 19) KEGG pathways were significantly enriched after submergence and post-submergence recovery in roots (shoots), including plant hormone signaling, sulfur metabolism, carbohydrate metabolism and phenylpropanoid biosynthesis. Moreover, protein-protein interaction (PPI) network was conducted, several hub genes were identified, including those encoding ribosomal proteins, translation initiation factor, persulfide dioxygenase ETHE1 and Enolase 1. Finally, a peroxidase gene CdPER47 were selected and cloned, CdPER47 exhibited significantly differential expression in roots under both submergence and post-submergence stresses. Heterogeneous overexpression of CdPER47 in Arabidopsis increased its survival rate significantly after submergence stress. Based on the above results, a hypothetical model was proposed for bermudagrass responses to submergence and post-submergence stresses. Our study reveals that bermudagrass responds to submergence and post-submergence stresses through extensive transcriptional reprogramming involving AP2-EREBP transcription factors, multiple metabolic pathways, and key hub genes including the functionally validated peroxidase gene CdPER47. Together, this study will provide valuable references for developing submergence-tolerance germplasm in the future.