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Background <p><i>Perilla frutescens</i> is an oilseed crop rich in unsaturated fatty acids (UFAs). WRINKLED1 (WRI1) is an AP2/EREBP-type transcription factor (TF), which plays important regulation roles in fatty acid (FA) metabolism.</p> Results <p>In this study, 11 PfWRI1 members were identified in the perilla genome. qRT-PCR analysis revealed that <i>PfWRI1-10</i> was expressed higher than other members in developing seeds of perilla, suggesting that <i>PfWRI1-10</i> may play crucial roles in seed oil biosynthesis. Overexpression of <i>PfWRI1-10</i> resulted in enhancement of total oil content by 9.75% and α-linolenic acid (C18:3) level by 0.43%, while the soluble sugar content decreased in the transgenic tobacco leaves. qRT-PCR showed that the expression levels of glycolysis gene (<i>NtPKp-β1</i>) and FA biosynthesis genes (<i>NtACP1</i> and <i>NtBCCP1</i>) significantly upregulated, whereas <i>NtKAS1-B</i> expression was downregulated in transgenic tobacco plants, indicating that <i>PfWRI1-10</i> reallocated carbon sources flow to facilitate lipid biosynthesis by regulating the expression of the target genes. Yeast one-hybrid (Y1H) assays demonstrate that PfWRI1-10 directly binds to the AW-box <i>cis</i>-element of the <i>PfBCCP1</i> promoter. Overexpression of <i>PfBCCP1</i> enhanced the lipid content in the transgenic yeast, confirming its function in lipid metabolism.</p> Conclusions <p>PfWRI1-10 transcription factor promotes lipid biosynthesis by upregulating <i>PfBCCP1</i> and other lipid related genes. The current findings provide a scientific basis for the in-depth analysis of the regulatory network of perilla oil biosynthesis and also valuable genes for genetic improvement of perilla oil yield and quality.</p>

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PfWRI1-10 transcription factor mediates lipid synthesis in Perilla frutescens through regulating PfBCCP1 gene transcription

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月6日

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  • Xudong Chai,
  • Yali Zhou,
  • Jing Wen,
  • Ting Lei,
  • Hongli Yang,
  • Miao Yin,
  • Xusheng Huang,
  • Rui Li,
  • Runzhi Li,
  • Jiping Wang

摘要

Background

Perilla frutescens is an oilseed crop rich in unsaturated fatty acids (UFAs). WRINKLED1 (WRI1) is an AP2/EREBP-type transcription factor (TF), which plays important regulation roles in fatty acid (FA) metabolism.

Results

In this study, 11 PfWRI1 members were identified in the perilla genome. qRT-PCR analysis revealed that PfWRI1-10 was expressed higher than other members in developing seeds of perilla, suggesting that PfWRI1-10 may play crucial roles in seed oil biosynthesis. Overexpression of PfWRI1-10 resulted in enhancement of total oil content by 9.75% and α-linolenic acid (C18:3) level by 0.43%, while the soluble sugar content decreased in the transgenic tobacco leaves. qRT-PCR showed that the expression levels of glycolysis gene (NtPKp-β1) and FA biosynthesis genes (NtACP1 and NtBCCP1) significantly upregulated, whereas NtKAS1-B expression was downregulated in transgenic tobacco plants, indicating that PfWRI1-10 reallocated carbon sources flow to facilitate lipid biosynthesis by regulating the expression of the target genes. Yeast one-hybrid (Y1H) assays demonstrate that PfWRI1-10 directly binds to the AW-box cis-element of the PfBCCP1 promoter. Overexpression of PfBCCP1 enhanced the lipid content in the transgenic yeast, confirming its function in lipid metabolism.

Conclusions

PfWRI1-10 transcription factor promotes lipid biosynthesis by upregulating PfBCCP1 and other lipid related genes. The current findings provide a scientific basis for the in-depth analysis of the regulatory network of perilla oil biosynthesis and also valuable genes for genetic improvement of perilla oil yield and quality.