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Background <p><i>Panax notoginseng</i> is widely cultivated in China because of its medicinal properties. However, <i>P. notoginseng</i> is susceptible to various diseases during its cultivation. Salicylic acid (SA) plays a pivotal role in the response to various biotic stresses by mediating powerful plant immunity. In recent years, the role of the NPR-TGA module as an activator of SA-mediated defence responses has been widely investigated. Although the NPR-TGA module has been extensively studied in many model species, information on the NPR-TGA module in <i>P. notoginseng</i> is lacking.</p> Results <p>To elucidate their functions, 6 <i>PnNPR</i> genes and 10 <i>PnTGA</i> genes with conserved domains and different physicochemical characteristics were identified in the genome of <i>P. notoginseng</i>. The <i>PnNPR</i>-<i>PnTGA</i> modules were divided into 3 subgroups and 5 subgroups according to their phylogenetic relationship. In this study, the 2000&#xa0;bp promoter region was examined, and the types and numbers of <i>cis</i>-elements of each <i>PnNPR</i>-<i>PnTGA</i> module were identified. Notably, after SA treatment, PnNPR1 translocated from the cytoplasm to the nucleus indicating that it acts as an SA receptor in <i>P. notoginseng</i> during SA-mediated immune responses. Moreover, RT-qPCR analysis of different tissues revealed the potentially involvement of these modules in the development of different tissues. Additionally, RNA-seq analysis under salicylic acid, methyl jasmonate and melatonin treatment also suggested their wide involvement in plant hormone signalling transmission. Specifically, <i>PnNPR1</i> was significantly upregulated after 4&#xa0;h of MeJA and MT treatment, indicating that PnNPR1 plays a pivotal role in immunity by regulating the crosstalk between SA and MT/JA. Finally, the interaction between PnTGA2 and PnNPR1 was confirmed in this study.</p> Conclusions <p>In summary, this study enhances our understanding of the PnNPR-PnTGA module in <i>P. notoginseng</i>, revealing the potential role of PnNPR-PnTGA in response to biotic stresses. These findings provide valuable insight to guide future research on <i>PnNPR-PnTGA</i> genes modules and provide a reference for agricultural breeding applications.</p>

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Genome-wide identification and expression analysis of the PnNPR-PnTGA gene family reveal its potential role in response to biotic stresses in Panax notoginseng

  • 期刊论文

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  • 发表日期：

    2026年4月24日

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  • Leilin Li,
  • Jianbin Li,
  • Tiantai Liu,
  • Jiae Hou,
  • Mingtao Ai,
  • Xiuming Cui,
  • Qian Yang

摘要

Background

Panax notoginseng is widely cultivated in China because of its medicinal properties. However, P. notoginseng is susceptible to various diseases during its cultivation. Salicylic acid (SA) plays a pivotal role in the response to various biotic stresses by mediating powerful plant immunity. In recent years, the role of the NPR-TGA module as an activator of SA-mediated defence responses has been widely investigated. Although the NPR-TGA module has been extensively studied in many model species, information on the NPR-TGA module in P. notoginseng is lacking.

Results

To elucidate their functions, 6 PnNPR genes and 10 PnTGA genes with conserved domains and different physicochemical characteristics were identified in the genome of P. notoginseng. The PnNPR-PnTGA modules were divided into 3 subgroups and 5 subgroups according to their phylogenetic relationship. In this study, the 2000 bp promoter region was examined, and the types and numbers of cis-elements of each PnNPR-PnTGA module were identified. Notably, after SA treatment, PnNPR1 translocated from the cytoplasm to the nucleus indicating that it acts as an SA receptor in P. notoginseng during SA-mediated immune responses. Moreover, RT-qPCR analysis of different tissues revealed the potentially involvement of these modules in the development of different tissues. Additionally, RNA-seq analysis under salicylic acid, methyl jasmonate and melatonin treatment also suggested their wide involvement in plant hormone signalling transmission. Specifically, PnNPR1 was significantly upregulated after 4 h of MeJA and MT treatment, indicating that PnNPR1 plays a pivotal role in immunity by regulating the crosstalk between SA and MT/JA. Finally, the interaction between PnTGA2 and PnNPR1 was confirmed in this study.

Conclusions

In summary, this study enhances our understanding of the PnNPR-PnTGA module in P. notoginseng, revealing the potential role of PnNPR-PnTGA in response to biotic stresses. These findings provide valuable insight to guide future research on PnNPR-PnTGA genes modules and provide a reference for agricultural breeding applications.