Scholarlink

Background <p>Plant endoglucanases of glycosyl hydrolase family 9 (GH9), which cleave 1,4-β-glucosidic bonds in glycan chains, have been shown to play pivotal roles in cellulose degradation, as well as in the relaxation or construction of cell walls during diverse plant growth and developmental processes. However, their specific functions in plant sexual reproduction remain unclear.</p> Results <p>In this study, we systematically analyzed expression profiles of the <i>GH9</i> gene family using in vivo observations of GFP-translational fusion proteins. Our results revealed that numerous <i>GH9</i> genes were expressed across both vegetative and reproductive tissues: 5 in stomata, 12 in roots, 11 in mature pollen and 15 in mature ovules or seeds. Further analysis of reproductive tissues uncovered distinct expression specificities: <i>GH9A4</i> as pollen-specific, <i>GH9B6</i> as sperm cell-specific, <i>GH9B12</i> as central cell- and endosperm cell-specific, <i>GH9B11</i> as enriched in pollen and chalazal endosperm, and <i>GH9B8</i> as vascular tissue-preferential. Additionally, GFP signals of up to 7 GH9 members were detected in the filiform apparatus, while 12 GH9 members showed signals in integuments and seed coats—hinting at diverse GH9 functions in reproduction. Furthermore, we conducted gene editing and phenotypic analysis on two subsets of <i>GH9</i> genes highly expressed in pollen. While in vivo pollen germination and growth were unaffected, in vitro germination rates decreased significantly when <i>GH9B5</i>, <i>GH9B7</i>, and <i>GH9A4</i> functionally deficient.</p> Conclusions <p>Taken together, this study highlights the potential roles of the GH9 family in pollen germination and establishes a foundation for future functional analyses aimed at elucidating the putative roles of the GH9 family in plant reproduction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Expression patterns of Arabidopsis endo-β-1,4-glucanases and their putative roles in sexual reproduction

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月26日

查看全文
  • Han-xian Xiong,
  • Xiao-di Gao,
  • Wei Wang

摘要

Background

Plant endoglucanases of glycosyl hydrolase family 9 (GH9), which cleave 1,4-β-glucosidic bonds in glycan chains, have been shown to play pivotal roles in cellulose degradation, as well as in the relaxation or construction of cell walls during diverse plant growth and developmental processes. However, their specific functions in plant sexual reproduction remain unclear.

Results

In this study, we systematically analyzed expression profiles of the GH9 gene family using in vivo observations of GFP-translational fusion proteins. Our results revealed that numerous GH9 genes were expressed across both vegetative and reproductive tissues: 5 in stomata, 12 in roots, 11 in mature pollen and 15 in mature ovules or seeds. Further analysis of reproductive tissues uncovered distinct expression specificities: GH9A4 as pollen-specific, GH9B6 as sperm cell-specific, GH9B12 as central cell- and endosperm cell-specific, GH9B11 as enriched in pollen and chalazal endosperm, and GH9B8 as vascular tissue-preferential. Additionally, GFP signals of up to 7 GH9 members were detected in the filiform apparatus, while 12 GH9 members showed signals in integuments and seed coats—hinting at diverse GH9 functions in reproduction. Furthermore, we conducted gene editing and phenotypic analysis on two subsets of GH9 genes highly expressed in pollen. While in vivo pollen germination and growth were unaffected, in vitro germination rates decreased significantly when GH9B5, GH9B7, and GH9A4 functionally deficient.

Conclusions

Taken together, this study highlights the potential roles of the GH9 family in pollen germination and establishes a foundation for future functional analyses aimed at elucidating the putative roles of the GH9 family in plant reproduction.