Scholarlink

Background <p>Phosphorus (P) deficiency is a major constraint on soybean (<i>Glycine max</i> (L.) Merr.) productivity, a key global source of edible oil and protein. Enhancing low-phosphorus (LP) tolerance through improved phosphorus-use efficiency is therefore crucial for sustainable soybean cultivation.</p> Results <p>This study elucidates the molecular mechanism by which <i>GmAux/IAA16</i> regulates root morphogenesis and coordinates LP responses. Bioinformatics analysis showed that GmAux/IAA16 shares 92.43% sequence identity with its wild soybean (<i>Glycine soja</i>) ortholog, and subcellular localization confirmed its nuclear-specific accumulation. Stable overexpression of <i>GmAux/IAA16</i> in tobacco via <i>Agrobacterium</i>-mediated transformation enhanced root development and maintained physiological stability under phosphate deprivation. Under LP stress, transgenic plants exhibited significant increases in key root enzyme activities: increased by 27.75% for indole‑3‑acetic acid oxidase (IAAO), 11.99% for superoxide dismutase (SOD), 42.59% for acid phosphatase (ACP), and 123.55% for catalase (CAT), alongside elevated ATP levels, reduced malondialdehyde (MDA) accumulation, and increased proline (Pro) content. Furthermore, endogenous levels of indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid (SA) were significantly higher in both leaves and roots, indicating a coordinated hormonal response that facilitates LP adaptation. Transcriptome analysis revealed that <i>GmAux/IAA16</i> overexpression substantially reshapes the transcriptional landscape of tobacco roots under phosphorus stress.</p> Conclusion <p>These findings demonstrate that <i>GmAux/IAA16</i> positively regulates low-phosphorus tolerance, likely through cascading regulation of downstream phosphate-starvation response genes. This study provides novel insights into the role of the <i>Aux/IAA</i> gene family in soybean root development and phosphorus-use efficiency, and identifies <i>GmAux/IAA16</i> as a promising genetic target for molecular breeding of phosphorus-efficient soybean cultivars.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

GmAux/IAA16 exerts a positive regulatory role in enhancing low-phosphorus stress tolerance in plants

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月17日

查看全文
  • Yunyan Lu,
  • Yaqiong Zhang,
  • Rong Li,
  • Xiaoai Yang,
  • Yunyue Du,
  • Ting Zhang,
  • Yinjia Xiang,
  • Liang Wang,
  • Quan Liang

摘要

Background

Phosphorus (P) deficiency is a major constraint on soybean (Glycine max (L.) Merr.) productivity, a key global source of edible oil and protein. Enhancing low-phosphorus (LP) tolerance through improved phosphorus-use efficiency is therefore crucial for sustainable soybean cultivation.

Results

This study elucidates the molecular mechanism by which GmAux/IAA16 regulates root morphogenesis and coordinates LP responses. Bioinformatics analysis showed that GmAux/IAA16 shares 92.43% sequence identity with its wild soybean (Glycine soja) ortholog, and subcellular localization confirmed its nuclear-specific accumulation. Stable overexpression of GmAux/IAA16 in tobacco via Agrobacterium-mediated transformation enhanced root development and maintained physiological stability under phosphate deprivation. Under LP stress, transgenic plants exhibited significant increases in key root enzyme activities: increased by 27.75% for indole‑3‑acetic acid oxidase (IAAO), 11.99% for superoxide dismutase (SOD), 42.59% for acid phosphatase (ACP), and 123.55% for catalase (CAT), alongside elevated ATP levels, reduced malondialdehyde (MDA) accumulation, and increased proline (Pro) content. Furthermore, endogenous levels of indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid (SA) were significantly higher in both leaves and roots, indicating a coordinated hormonal response that facilitates LP adaptation. Transcriptome analysis revealed that GmAux/IAA16 overexpression substantially reshapes the transcriptional landscape of tobacco roots under phosphorus stress.

Conclusion

These findings demonstrate that GmAux/IAA16 positively regulates low-phosphorus tolerance, likely through cascading regulation of downstream phosphate-starvation response genes. This study provides novel insights into the role of the Aux/IAA gene family in soybean root development and phosphorus-use efficiency, and identifies GmAux/IAA16 as a promising genetic target for molecular breeding of phosphorus-efficient soybean cultivars.