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Background <p>Nodule inception-like Protein (<i>NLP</i>) family genes, as transcription factors, play an important role in regulating physiological responses of plants to adapt to external nitrogen environment changes. However, there are few reports on <i>NLP</i> genes in cucumber.</p> Results <p>In this study, we identified members of the cucumber family, conducted a comprehensive bioinformatics analysis of them, analyzed their evolutionary relationships, and predicted their potential functions. The results showed that there were 3 homologous genes in cucumber. The evolutionary analysis of NLPs in different species showed that the NLP family proteins mostly contained three conserved domains: RWP-RK, PB-1 and GAF-like domains. All of them contained four introns and five exons, and the motif composition and protein structure were similar. By analyzing the promoter region, it was found that the cucumber <i>NLP</i> gene family contains cis-acting elements related to plant growth, environmental stress and hormone signaling, suggesting that this gene family may be involved in the growth and development of cucumber under stress and stress response. The cucumber <i>NLP</i> family genes were expressed in root, stem, leaf, flower, NLP and fruit tissues, and the <i>CsNLP2</i> gene showed the highest expression in leaves. Interestingly, family genes respond to both nitrogen environment changesand abiotic stresses, especially <i>CsNLP2</i>. Further subcellular localization analysis showed that CsNLP2 was mainly located in the nucleus. The CsNLP2 transcription factor positively regulates the expression of <i>CsNRT2.3</i> gene, which encodes the nitrate transporter. Overall, <i>CsNLP2</i> was a strong candidate gene for improving nitrogen use efficiency in cucumber.</p>

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Identification and expression characteristics of NIN-like protein (NLP) gene family in cucumber plant (Cucumis sativus L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月30日

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  • Yang Li,
  • Yijing Xing,
  • Ruohan Jin,
  • Luyu Li,
  • Mengwei Huang,
  • Meng Li,
  • Dandan Cui,
  • Huaijuan Xiao,
  • Shengli Li,
  • Juanqi Li

摘要

Background

Nodule inception-like Protein (NLP) family genes, as transcription factors, play an important role in regulating physiological responses of plants to adapt to external nitrogen environment changes. However, there are few reports on NLP genes in cucumber.

Results

In this study, we identified members of the cucumber family, conducted a comprehensive bioinformatics analysis of them, analyzed their evolutionary relationships, and predicted their potential functions. The results showed that there were 3 homologous genes in cucumber. The evolutionary analysis of NLPs in different species showed that the NLP family proteins mostly contained three conserved domains: RWP-RK, PB-1 and GAF-like domains. All of them contained four introns and five exons, and the motif composition and protein structure were similar. By analyzing the promoter region, it was found that the cucumber NLP gene family contains cis-acting elements related to plant growth, environmental stress and hormone signaling, suggesting that this gene family may be involved in the growth and development of cucumber under stress and stress response. The cucumber NLP family genes were expressed in root, stem, leaf, flower, NLP and fruit tissues, and the CsNLP2 gene showed the highest expression in leaves. Interestingly, family genes respond to both nitrogen environment changesand abiotic stresses, especially CsNLP2. Further subcellular localization analysis showed that CsNLP2 was mainly located in the nucleus. The CsNLP2 transcription factor positively regulates the expression of CsNRT2.3 gene, which encodes the nitrate transporter. Overall, CsNLP2 was a strong candidate gene for improving nitrogen use efficiency in cucumber.