Scholarlink

<p>The lack of a high-quality <i>Ulmus parvifolia</i> genome assembly has impeded research on disease resistance and hindered breeding programs for resilient elm cultivars. In this study, we presented a chromosome-level genome assembly of <i>U. parvifolia</i> using integrated sequencing technologies. LTR retrotransposons, predominantly the Ty3/Gypsy Retand subfamily, underwent recent large-scale amplification, potentially contributing to the genome size of <i>U. parvifolia</i>. Phylogenetically, <i>U. parvifolia</i> is sister to a clade containing <i>Moraceae</i>, <i>Cannabaceae</i>, and <i>Urticaceae</i>. Notably, gene families associated with disease resistance and immune response were significantly expanded in <i>U. parvifolia</i>, pointing to an adaptive evolution to various biotic and abiotic stresses. To dissect differential susceptibility to Dutch elm disease, we inoculated <i>U. parvifolia</i> (resistant) and <i>U. americana</i> (susceptible) with <i>Ceratocystis ulmi</i> and performed comparative transcriptomics at 48, 96, and 144&#xa0;h post-inoculation (hpi). Several plant defense and immune response pathways were markedly upregulated in <i>U. parvifolia</i> at 48/96 hpi, revealing a genetic basis for its enhanced resistance. This study advances genomic resources for elms, elucidates molecular mechanisms of disease resilience, and provides a foundation for breeding and conservation efforts.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chromosome-level genome assembly of Ulmus parvifolia identifies genomic signatures and transcriptional profiling underlying Dutch elm disease resistance

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月26日

查看全文
  • Yunzhou Lyu,
  • Hainan Sun,
  • Ruichang Yan,
  • Jiangtao Shi,
  • Libin Huang,
  • Gang Wang,
  • Lianyu Lin,
  • Jisen Zhang,
  • Wei Xing,
  • Xiaoyun Dong

摘要

The lack of a high-quality Ulmus parvifolia genome assembly has impeded research on disease resistance and hindered breeding programs for resilient elm cultivars. In this study, we presented a chromosome-level genome assembly of U. parvifolia using integrated sequencing technologies. LTR retrotransposons, predominantly the Ty3/Gypsy Retand subfamily, underwent recent large-scale amplification, potentially contributing to the genome size of U. parvifolia. Phylogenetically, U. parvifolia is sister to a clade containing Moraceae, Cannabaceae, and Urticaceae. Notably, gene families associated with disease resistance and immune response were significantly expanded in U. parvifolia, pointing to an adaptive evolution to various biotic and abiotic stresses. To dissect differential susceptibility to Dutch elm disease, we inoculated U. parvifolia (resistant) and U. americana (susceptible) with Ceratocystis ulmi and performed comparative transcriptomics at 48, 96, and 144 h post-inoculation (hpi). Several plant defense and immune response pathways were markedly upregulated in U. parvifolia at 48/96 hpi, revealing a genetic basis for its enhanced resistance. This study advances genomic resources for elms, elucidates molecular mechanisms of disease resilience, and provides a foundation for breeding and conservation efforts.