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Background <p>Ethylene (C<sub>2</sub>H<sub>4</sub>) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also considered as a plant growth regulator. Accurate quantification of ACC is critically important in investigating its function. However, it remains challenging to accurately quantify ACC in plant tissues because it is a small, electroneutral molecule with very low concentrations.</p> Methods <p>An easy, cost-saving, and highly efficient quantitative method for ACC in plant tissues was set up by liquid-liquid micro-extraction (LLME) purification with green solvent ethyl-acetate, and the precise control of the mobile phase entering into the mass spectrometer combined with the ultra-high performance liquid chromatography electrospray ionization-triple quadrupole mass spectrometer (UHPLC-ESI-MS/MS).</p> Results <p>The contents of ACC in 10&#xa0;mg of different fresh fruits were detected. The established method had limit of detection (LOD) (2.5 pg), matrix effect (ME) (92.6%), good precision (3.54%), recovery rate (95.82%), and a good linear relationship within the range of 0.5 to 1500 ng.mL<sup>−1</sup> (R<sup>2</sup> = 0.9998).</p> Conclusion <p>We have developed an easy, sensitive, and cost-saving quantitative method for ACC in plant tissues without derivatization which is useful for the precise quantification of ACC in plant tissues.</p>

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Quantitative analysis of ethylene precursor ACC in plant samples by liquid chromatography-tandem mass spectrometry

  • 期刊论文

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  • 发表日期：

    2025年7月17日

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  • Jianhua Tong,
  • Wenkui Zhao,
  • Keming Wang,
  • Feiying Zhu,
  • Danyi Deng,
  • Langtao Xiao

摘要

Background

Ethylene (C2H4) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also considered as a plant growth regulator. Accurate quantification of ACC is critically important in investigating its function. However, it remains challenging to accurately quantify ACC in plant tissues because it is a small, electroneutral molecule with very low concentrations.

Methods

An easy, cost-saving, and highly efficient quantitative method for ACC in plant tissues was set up by liquid-liquid micro-extraction (LLME) purification with green solvent ethyl-acetate, and the precise control of the mobile phase entering into the mass spectrometer combined with the ultra-high performance liquid chromatography electrospray ionization-triple quadrupole mass spectrometer (UHPLC-ESI-MS/MS).

Results

The contents of ACC in 10 mg of different fresh fruits were detected. The established method had limit of detection (LOD) (2.5 pg), matrix effect (ME) (92.6%), good precision (3.54%), recovery rate (95.82%), and a good linear relationship within the range of 0.5 to 1500 ng.mL−1 (R2 = 0.9998).

Conclusion

We have developed an easy, sensitive, and cost-saving quantitative method for ACC in plant tissues without derivatization which is useful for the precise quantification of ACC in plant tissues.