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Background <p><i>Leonurus japonicus</i> (<i>L. japonicus</i>) is a herbaceous flowering plant, widely distributed in Asia. Drought is one of the primary environmental stress factors affecting <i>L. japonicus</i> growth. Previous studies have demonstrated that WRKY transcription factors (TFs) play a crucial role in plant responses to drought stress. So far, there has been no research on the function of WRKY genes in <i>L. japonicus</i>.</p> Results <p>The physiological experiment results showed that drought stress significantly increased the malondialdehyde (MDA), proline, and hydrogen peroxide (H₂O₂) content of <i>L. japonicus</i>. Transcriptome analysis revealed significant changes in the expression levels of the WRKY gene family. Based on bioinformatics analysis, 67 WRKY genes (<i>LjWRKYs</i>) were identified in the genome of <i>L. japonicus</i>, with amino acid lengths ranging from 85 to 574. The <i>LjWRKYs</i> can be divided into three subfamilies. Among them, the expression of <i>LjWRKY</i> (1/4/23/44) were significantly up-regulated under drought stress, whereas the expression of <i>LjWRKY</i> (21/25/65) were significantly down-regulated. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that after drought stress, differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathway, the MAPK signaling pathway and biosynthesis of secondary metabolites pathway. In the MAPK pathway, there were 19 DEGs, 9 of which contained W-box regions, suggesting that they may be potential regulatory targets of LjWRKY TFs under drought stress.</p> Conclusion <p>These findings suggested that WRKY gene family may participate in the response to drought stress in <i>L. japonicus</i>. This study provides a scientific basis for the further development and functional validation of the WRKY gene family in <i>L. japonicus.</i></p> Clinical trial number <p>Not applicable.</p>

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Transcriptome analysis and genome-wide identification of WRKY gene family in Leonurus japonicus under drought stress

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月9日

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  • Tongtong Guo,
  • Qing Wang,
  • Yuxiao Yi,
  • Zelin Yin,
  • Lili Xu,
  • Fengtang Yang,
  • Jianing Xu,
  • Ming Zhang

摘要

Background

Leonurus japonicus (L. japonicus) is a herbaceous flowering plant, widely distributed in Asia. Drought is one of the primary environmental stress factors affecting L. japonicus growth. Previous studies have demonstrated that WRKY transcription factors (TFs) play a crucial role in plant responses to drought stress. So far, there has been no research on the function of WRKY genes in L. japonicus.

Results

The physiological experiment results showed that drought stress significantly increased the malondialdehyde (MDA), proline, and hydrogen peroxide (H₂O₂) content of L. japonicus. Transcriptome analysis revealed significant changes in the expression levels of the WRKY gene family. Based on bioinformatics analysis, 67 WRKY genes (LjWRKYs) were identified in the genome of L. japonicus, with amino acid lengths ranging from 85 to 574. The LjWRKYs can be divided into three subfamilies. Among them, the expression of LjWRKY (1/4/23/44) were significantly up-regulated under drought stress, whereas the expression of LjWRKY (21/25/65) were significantly down-regulated. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that after drought stress, differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathway, the MAPK signaling pathway and biosynthesis of secondary metabolites pathway. In the MAPK pathway, there were 19 DEGs, 9 of which contained W-box regions, suggesting that they may be potential regulatory targets of LjWRKY TFs under drought stress.

Conclusion

These findings suggested that WRKY gene family may participate in the response to drought stress in L. japonicus. This study provides a scientific basis for the further development and functional validation of the WRKY gene family in L. japonicus.

Clinical trial number

Not applicable.