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Background <p>Flavonoid biosynthesis pathway is generally thought unique to land plants and has assisted plants to adapt the terrestrial ecosystems. In this pathway, four 2-oxoglutarate/Fe(II)-dependent dioxygenases (2-ODDs), i.e., flavone synthase I (FNSI), flavanone-3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanin synthase/leucoanthocyanidin dioxygenase (ANS/LDOX), catalyze the hydroxylation and desaturation reactions. In bryophytes, the earliest land plant group, little is known about the biological functions of these enzymes.</p> Results <p>Here, we cloned a <i>Pn2-ODD2</i> gene of flavonoid biosynthesis pathway from Antarctic moss <i>Pohlia nutans</i>, which was induced by exogenous NaCl, PEG and abscisic acid (ABA) treatment. Overexpression of <i>Pn2-ODD2</i> increased the drought resistance in <i>Physcomitrium patens</i> and <i>Arabidopsis thaliana</i> during gametophyte growth and seed germination, respectively. Overexpressed-<i>Pn2-ODD2 Arabidopsis</i> also exhibited the enhanced tolerance to oxidative stress, with the downregulation of ROS generation gene and increased the total flavonoid content. Also, overexpression of <i>Pn2-ODD2</i> decreased the ABA sensitivity in transgenic <i>P. patens</i> and <i>Arabidopsis</i>. Meanwhile, overexpression of <i>Pn2-ODD2</i> resulted in an increase in both anthocyanins and flavonols in <i>Arabidopsis</i>, which was correlated with the up-regulated anthocyanin biosynthesis gene.</p> Conclusions <p>Taken together, <i>Pn2-ODD2</i> conferred the resistance to drought and oxidative stress by regulating antioxidant defense system in plants.</p>

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The Antarctic moss 2-oxoglutarate/Fe(II)-dependent dioxygenases (Pn2-ODD2) enhanced the tolerance to drought and oxidative stress

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月28日

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  • Huijuan Wang,
  • Han Li,
  • Chaochao Li,
  • Shenghao Liu,
  • Pengying Zhang

摘要

Background

Flavonoid biosynthesis pathway is generally thought unique to land plants and has assisted plants to adapt the terrestrial ecosystems. In this pathway, four 2-oxoglutarate/Fe(II)-dependent dioxygenases (2-ODDs), i.e., flavone synthase I (FNSI), flavanone-3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanin synthase/leucoanthocyanidin dioxygenase (ANS/LDOX), catalyze the hydroxylation and desaturation reactions. In bryophytes, the earliest land plant group, little is known about the biological functions of these enzymes.

Results

Here, we cloned a Pn2-ODD2 gene of flavonoid biosynthesis pathway from Antarctic moss Pohlia nutans, which was induced by exogenous NaCl, PEG and abscisic acid (ABA) treatment. Overexpression of Pn2-ODD2 increased the drought resistance in Physcomitrium patens and Arabidopsis thaliana during gametophyte growth and seed germination, respectively. Overexpressed-Pn2-ODD2 Arabidopsis also exhibited the enhanced tolerance to oxidative stress, with the downregulation of ROS generation gene and increased the total flavonoid content. Also, overexpression of Pn2-ODD2 decreased the ABA sensitivity in transgenic P. patens and Arabidopsis. Meanwhile, overexpression of Pn2-ODD2 resulted in an increase in both anthocyanins and flavonols in Arabidopsis, which was correlated with the up-regulated anthocyanin biosynthesis gene.

Conclusions

Taken together, Pn2-ODD2 conferred the resistance to drought and oxidative stress by regulating antioxidant defense system in plants.