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Background <p>Aerenchyma in adventitious roots plays a crucial role in rice growth under flooding conditions. However, the mechanisms underlying the dynamic formation process of aerenchyma remain poorly understood, largely due to the time-consuming and labor-intensive nature of traditional sectioning methods for analyzing aerenchyma formation. In this study, we optimized the Gompertz model to investigate the dynamic process of aerenchyma formation and its relationship with root growth. The materials used included the wild-type <i>Nipponbare</i> (NIP) as well as <i>OsRGA1</i> knockout and overexpression lines, <i>rga1–</i>5 and <i>RGA1–</i>OE1.</p> Results <p>All three parameters of the optimized U<i>-</i>Gompertz model directly characterized the dynamic process of aerenchyma formation. Compared to NIP, the <i>rga1–</i>5 knockout line exhibited significantly lower maximum value and rate of aerenchyma formation, with delays at the initial point, maximum acceleration point, inflection point, maximum deceleration point, and maximum value point. In contrast, <i>RGA1</i>–OE1 line displayed an opposite trend. Additionally, <i>OsRGA1</i> upregulated <i>RBOH</i> gene expression, reduced catalase and peroxidase activities, and consequently increased the endogenous H<sub>2</sub>O<sub>2</sub> content from the initial point to the inflection point of aerenchyma formation.</p> Conclusions <p>As evidenced by the optimized U-Gompertz model, our results demonstrate that <i>OsRGA1</i> accelerates aerenchyma formation by increasing H<sub>2</sub>O<sub>2</sub> levels, thereby enhancing root activity and promoting root growth in rice.</p>

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The role of OsRGA1 in aerenchyma formation and adventitious root growth in rice seedlings based on the U-Gompertz model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月17日

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  • Jiamei Zhao,
  • Dongyao Wang,
  • Yujie Tang,
  • Huan Yu,
  • Yani Zhang,
  • Li Zhu,
  • Xinyao Yan,
  • Jiqiang Yang,
  • Lijun Liu,
  • Yun Chen

摘要

Background

Aerenchyma in adventitious roots plays a crucial role in rice growth under flooding conditions. However, the mechanisms underlying the dynamic formation process of aerenchyma remain poorly understood, largely due to the time-consuming and labor-intensive nature of traditional sectioning methods for analyzing aerenchyma formation. In this study, we optimized the Gompertz model to investigate the dynamic process of aerenchyma formation and its relationship with root growth. The materials used included the wild-type Nipponbare (NIP) as well as OsRGA1 knockout and overexpression lines, rga1–5 and RGA1–OE1.

Results

All three parameters of the optimized U-Gompertz model directly characterized the dynamic process of aerenchyma formation. Compared to NIP, the rga1–5 knockout line exhibited significantly lower maximum value and rate of aerenchyma formation, with delays at the initial point, maximum acceleration point, inflection point, maximum deceleration point, and maximum value point. In contrast, RGA1–OE1 line displayed an opposite trend. Additionally, OsRGA1 upregulated RBOH gene expression, reduced catalase and peroxidase activities, and consequently increased the endogenous H2O2 content from the initial point to the inflection point of aerenchyma formation.

Conclusions

As evidenced by the optimized U-Gompertz model, our results demonstrate that OsRGA1 accelerates aerenchyma formation by increasing H2O2 levels, thereby enhancing root activity and promoting root growth in rice.