Scholarlink

Background <p><i>Radix Bupleuri</i> is a popular traditional Chinese medicinal plant. Its root contains saikosaponin and volatile oil compounds with antipyretic, anti-inflammatory, and hepatoprotective pharmacological effects. However, there are differences in the content and type of main chemical components in the roots of three <i>Bupleurum</i> species: <i>Bupleurum chinense</i> DC. (Bchi), <i>Bupleurum scorzonerifolium</i> Willd. (Bsco), and <i>Bupleurum marginatum</i> var. <i>stenophyllum</i> (Wolff) Shan et Y.Li (Bmar). The molecular mechanism behind these differences is still unclear. The present study used integrated metabolome and transcriptome analyses to uncover the differences in metabolites and expressed genes among the three <i>Bupleurum</i> species.</p> Results <p>Metabolomics results revealed that Bmar contained more saikosaponins than Bchi and Bsco. Conversely, Bsco had the highest content of volatile oil monoterpenes but a lower sesquiterpene content than Bchi and Bmar. Transcriptome analysis showed that several genes were highly expressed in Bchi, Bsco, or Bmar, demonstrating the molecular mechanism responsible for the differences in their metabolic components. We combined the metabolomics and transcriptomics data to investigate the relationship between metabolites and genes. The results showed a high correlation between CYP450, UGT, and β-AS genes and 6’’-acetyl-saikosaponins A, saikosaponins B1, C, and D. The subcellular localization of the two P450 genes (<i>Bc087391</i> and <i>Bc036879</i>) in the endoplasmic reticulum suggests that they may be involved in saikosaponin biosynthesis.</p> Conclusion <p>We performed an integrated transcriptome and metabolome analysis to investigate the diversity of the terpenoid biosynthetic pathway in three <i>Bupleurum</i> species. The study provides new insights into the molecular basis of the metabolic differences between the three <i>Bupleurum</i> species. It also serves as a theoretical basis for the clinical application and breeding of <i>Bupleurum</i> resources.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated metabolomics and transcriptomics reveal differences in terpenoids and the molecular basis among the roots of three Bupleurum species

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月31日

查看全文
  • Dong Wen,
  • Hongliang Ji,
  • Mei Rong,
  • Yang Liu,
  • Jiemei Jiang,
  • Xinwei Guo,
  • Zhihui Gao,
  • Yanhong Xu,
  • Jianhe Wei

摘要

Background

Radix Bupleuri is a popular traditional Chinese medicinal plant. Its root contains saikosaponin and volatile oil compounds with antipyretic, anti-inflammatory, and hepatoprotective pharmacological effects. However, there are differences in the content and type of main chemical components in the roots of three Bupleurum species: Bupleurum chinense DC. (Bchi), Bupleurum scorzonerifolium Willd. (Bsco), and Bupleurum marginatum var. stenophyllum (Wolff) Shan et Y.Li (Bmar). The molecular mechanism behind these differences is still unclear. The present study used integrated metabolome and transcriptome analyses to uncover the differences in metabolites and expressed genes among the three Bupleurum species.

Results

Metabolomics results revealed that Bmar contained more saikosaponins than Bchi and Bsco. Conversely, Bsco had the highest content of volatile oil monoterpenes but a lower sesquiterpene content than Bchi and Bmar. Transcriptome analysis showed that several genes were highly expressed in Bchi, Bsco, or Bmar, demonstrating the molecular mechanism responsible for the differences in their metabolic components. We combined the metabolomics and transcriptomics data to investigate the relationship between metabolites and genes. The results showed a high correlation between CYP450, UGT, and β-AS genes and 6’’-acetyl-saikosaponins A, saikosaponins B1, C, and D. The subcellular localization of the two P450 genes (Bc087391 and Bc036879) in the endoplasmic reticulum suggests that they may be involved in saikosaponin biosynthesis.

Conclusion

We performed an integrated transcriptome and metabolome analysis to investigate the diversity of the terpenoid biosynthetic pathway in three Bupleurum species. The study provides new insights into the molecular basis of the metabolic differences between the three Bupleurum species. It also serves as a theoretical basis for the clinical application and breeding of Bupleurum resources.