Background <p>Wild relatives have substantial impacts on the resistance of wheat to biotic and abiotic stresses. The genetic diversity of these wild varieties can be employed to widen the wheat gene pool by introducing wild allele introgression and genome structure variations. The powdery mildew resistance gene <i>Pm13</i> was derived from the wheat wild relative species <i>Aegilops longissima</i> (S¹S¹, 2n = 2x = 14) and was transferred into wheat chromosome 3BS over 30 years. Until recently, it has not been widely used in wheat breeding, possibly because of the linkage drag associated with <i>Pm13</i> introgression or poor alien segment compensation to 3BS.</p> Results <p>In this study, translocations between the <i>Pm13</i> introgression and wheat homoeologous A, B and D chromosomes were developed by using a wheat <i>ph1b</i> mutant. The 3S^l (3B) substitution line was hybridized with the CS nullisomic-tetrasomic line N3AT3B, and then F₁ was hybridized with the <i>CSph1b</i> mutant. Individuals homozygous for the <i>ph1b</i> mutant carrying the alien chromosome 3S^l were identified from the segregating populations via molecular markers and cytological analysis. Whole-arm recombinant translocations T3S^lS.3AL and T3S^lS.3BL and small recombinant translocations T3S^lS-3AS.3AL, T3S^lS-3BS.3BL and T3DS-3S^lS-3DS.3DL carrying <i>Pm13</i> introgression were subsequently identified via molecular cytological analysis. Furthermore, 4 disomic T3S^lS-3BS.3BL recombinants carrying the homozygous <i>Pm13</i> introgression were identified. All the translocations were nearly immune to powdery mildew.</p> Conclusions <p>Our results provide novel alien homoeologous <i>Pm13</i> translocations for improving powdery mildew resistance in wheat and insights into the interactions between different wheat genomes and alien chromosome segments.</p>