Scholarlink

Background <p>Red <i>Actinidia arguta</i> has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in <i>A. arguta</i>.</p> Results <p>Here, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays a crucial role in <i>A. arguta</i> anthocyanin biosynthesis. The RT-qPCR results revealed that transient overexpression of <i>AaMYB61-like</i> in <i>A. arguta</i> fruit at 90–100 DAFB significantly promoted anthocyanin biosynthesis, as did the gene expression levels of <i>AaCHS, AaCHI, AaF3H, AaLDOX,</i> and <i>AaF3GT</i>, whereas the result of VIGS revealed the opposite results in <i>A. arguta</i> fruit at 105–115 DAFB. A transcriptional activation assay indicated that AaMYB61-like exhibited transcriptional activation activity. Y1H and LUC assays revealed that AaMYB61-like activates the promoters of <i>AaCHS, AaLDOX</i>, and <i>AaF3GT</i>. In addition, AabHLH137 was found to be related to fruit color from the transcriptome data. We demonstrated that AaMYB61-like promotes anthocyanin biosynthesis by interacting with AabHLH137 via Y2H, BiFC, and <i>Agrobacterium</i>-mediated co-transformation.</p> Conclusions <p>Our study not only reveals the functions of AaMYB61-like and AabHLH137 in anthocyanin regulation, but also broadly enriches color regulation theory, establishing a foundation for clarifying the molecular mechanism of fruit coloration in kiwifruit.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月22日

查看全文
  • Zhe Song,
  • Yukuo Li,
  • Xu Zhan,
  • Xiaohan Li,
  • Lingshuai Ye,
  • Miaomiao Lin,
  • Ran Wang,
  • Leiming Sun,
  • Jinyong Chen,
  • Jinbao Fang,
  • Feng Wei,
  • Xiujuan Qi

摘要

Background

Red Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in A. arguta.

Results

Here, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays a crucial role in A. arguta anthocyanin biosynthesis. The RT-qPCR results revealed that transient overexpression of AaMYB61-like in A. arguta fruit at 90–100 DAFB significantly promoted anthocyanin biosynthesis, as did the gene expression levels of AaCHS, AaCHI, AaF3H, AaLDOX, and AaF3GT, whereas the result of VIGS revealed the opposite results in A. arguta fruit at 105–115 DAFB. A transcriptional activation assay indicated that AaMYB61-like exhibited transcriptional activation activity. Y1H and LUC assays revealed that AaMYB61-like activates the promoters of AaCHS, AaLDOX, and AaF3GT. In addition, AabHLH137 was found to be related to fruit color from the transcriptome data. We demonstrated that AaMYB61-like promotes anthocyanin biosynthesis by interacting with AabHLH137 via Y2H, BiFC, and Agrobacterium-mediated co-transformation.

Conclusions

Our study not only reveals the functions of AaMYB61-like and AabHLH137 in anthocyanin regulation, but also broadly enriches color regulation theory, establishing a foundation for clarifying the molecular mechanism of fruit coloration in kiwifruit.