Scholarlink

Background <p>Gallstone disease is closely linked to gallbladder dysmotility and gut microbiota dysbiosis; however, the specific role of <i>Lactobacillus</i> depletion in this pathogenesis remains unclear. This study investigated whether lithogenic diet (LD) induced cholesterol gallstone formation is associated with <i>Lactobacillus</i> deficiency and evaluated the potential of Lactobacillus plantarum (L. plantarum) supplementation to mitigate stone formation by restoring gallbladder motility.</p> Methods and Results <p>Here, we used a murine cholelithiasis model using C57BL/6 mice fed an HFD to characterize whether gallstone formation in relation to gut microbiota dysbiosis and gallbladder dysmotility. <i>Lactobacillus</i> depletion was identified as a hallmark of gallstone formation, which was concomitant with gallbladder dysmotility. To target this deficit, <i>L. plantarum</i> was administered via daily gavage in an intervention cohort. <i>L. plantarum</i> supplementation significantly inhibited stone formation and improved CCK-induced gallbladder emptying. Histologically, probiotic treatment preserved gallbladder wall architecture and recovered α-SMA and interstitial Cajal-like cells (CLCs) expression levels compared to controls. These findings suggest that <i>Lactobacillus</i> deficiency may contribute to LD-induced cholelithiasis by impairing gallbladder motility, and that <i>L. plantarum</i> supplementation effectively prevents stone formation by restoring the gut microbiota–gallbladder motility axis.</p> Conclusion <p>LD-induced gallstone formation is characterized by <i>Lactobacillus</i> depletion and gallbladder dysmotility driven by the loss of smooth muscle integrity and CLCs. Supplementation with <i>L. plantarum</i> effectively prevents gallstone formation likely by restoring impaired gallbladder contractility. These findings highlight <i>Lactobacillus</i> as a promising non-invasive therapeutic strategy to prevent gallstones.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Lactobacillus plantarum may prevent cholesterol gallstone formation by restoring gallbladder motility through preserving Cajal-like cells in mice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月24日

查看全文
  • Lei Wang,
  • Xiao-Yu Liu,
  • Yi-Jie Sun,
  • Ze-Zhou Yang,
  • Ming-E Wu,
  • Meng-Run Kang,
  • Zhen-Kun Wang,
  • Jia-Wei Yu,
  • Yong Wan,
  • Tao Li,
  • Ying-Min Yao,
  • Yi Lv,
  • Qiang Lu

摘要

Background

Gallstone disease is closely linked to gallbladder dysmotility and gut microbiota dysbiosis; however, the specific role of Lactobacillus depletion in this pathogenesis remains unclear. This study investigated whether lithogenic diet (LD) induced cholesterol gallstone formation is associated with Lactobacillus deficiency and evaluated the potential of Lactobacillus plantarum (L. plantarum) supplementation to mitigate stone formation by restoring gallbladder motility.

Methods and Results

Here, we used a murine cholelithiasis model using C57BL/6 mice fed an HFD to characterize whether gallstone formation in relation to gut microbiota dysbiosis and gallbladder dysmotility. Lactobacillus depletion was identified as a hallmark of gallstone formation, which was concomitant with gallbladder dysmotility. To target this deficit, L. plantarum was administered via daily gavage in an intervention cohort. L. plantarum supplementation significantly inhibited stone formation and improved CCK-induced gallbladder emptying. Histologically, probiotic treatment preserved gallbladder wall architecture and recovered α-SMA and interstitial Cajal-like cells (CLCs) expression levels compared to controls. These findings suggest that Lactobacillus deficiency may contribute to LD-induced cholelithiasis by impairing gallbladder motility, and that L. plantarum supplementation effectively prevents stone formation by restoring the gut microbiota–gallbladder motility axis.

Conclusion

LD-induced gallstone formation is characterized by Lactobacillus depletion and gallbladder dysmotility driven by the loss of smooth muscle integrity and CLCs. Supplementation with L. plantarum effectively prevents gallstone formation likely by restoring impaired gallbladder contractility. These findings highlight Lactobacillus as a promising non-invasive therapeutic strategy to prevent gallstones.