Scholarlink

<p>Peanut early leaf spot, a major disease caused by <i>Passalora arachidicola</i>, is responsible for considerable yield reductions in peanut production. In this study, a highly antagonistic strain, <i>Bacillus amyloliquefaciens</i> TL6, was isolated and characterized from healthy peanut leaves. In vitro confrontation assays showed that strain TL6 produced an inhibition zone of 69.8&#xa0;mm against the pathogen. Pot experiments indicated that the undiluted fermentation broth of TL6 exhibited a biocontrol efficacy of 87.58% against peanut early leaf spot, with no statistical difference compared with 1000-fold diluted carbendazim (84.98%). Physiological and microscopic analyses revealed that the active metabolites from TL6 fermentation filtrate severely damaged <i>P. arachidicola</i> mycelia, causing mycelial wrinkling and fragmentation. Furthermore, the bioactive components of TL6 broth disrupted pathogen cell membrane homeostasis by inducing electrolyte leakage and suppressing ergosterol biosynthesis. Additionally, these active metabolites significantly increased the malondialdehyde content in <i>P. arachidicola</i>, but exhibited no significant effect on intracellular protein synthesis. Taken together, these results indicate that <i>B. amyloliquefaciens</i> TL6 has excellent potential as an effective biocontrol agent for the control of peanut early leaf spot disease.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Preliminary insight into the antifungal activity and mechanism of Bacillus amyloliquefaciens TL6 against Passalora arachidicola

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月31日

查看全文
  • Chaoqun Zang,
  • Ying Lin,
  • Chunhao Liang,
  • Yuanjun Bai

摘要

Peanut early leaf spot, a major disease caused by Passalora arachidicola, is responsible for considerable yield reductions in peanut production. In this study, a highly antagonistic strain, Bacillus amyloliquefaciens TL6, was isolated and characterized from healthy peanut leaves. In vitro confrontation assays showed that strain TL6 produced an inhibition zone of 69.8 mm against the pathogen. Pot experiments indicated that the undiluted fermentation broth of TL6 exhibited a biocontrol efficacy of 87.58% against peanut early leaf spot, with no statistical difference compared with 1000-fold diluted carbendazim (84.98%). Physiological and microscopic analyses revealed that the active metabolites from TL6 fermentation filtrate severely damaged P. arachidicola mycelia, causing mycelial wrinkling and fragmentation. Furthermore, the bioactive components of TL6 broth disrupted pathogen cell membrane homeostasis by inducing electrolyte leakage and suppressing ergosterol biosynthesis. Additionally, these active metabolites significantly increased the malondialdehyde content in P. arachidicola, but exhibited no significant effect on intracellular protein synthesis. Taken together, these results indicate that B. amyloliquefaciens TL6 has excellent potential as an effective biocontrol agent for the control of peanut early leaf spot disease.