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Background <p>Camels exhibit remarkable adaptability to harsh environments, in part due to their unique ruminal microbiota. <i>Paenibacillus barengoltzii</i>, isolated from camel rumen, plays a crucial role in rumen fermentation and plant biomass degradation. The present study aimed to identify fermentative genes and assess their lignocellulolytic potential.</p> Results <p>Whole-genome sequencing was performed on the Illumina MiSeq platform to enable comprehensive comparative genomic analysis of carbohydrate-active enzymes (CAZymes) across 11 <i>Paenibacillus</i> species. It was observed that species-specific CAZymes repertoires, with <i>P. barengoltzii</i> harboring genes for plant biomass degradation, including GH2, GH13, GH20, GH27, GH28, GH32, GH62, GH65, GH67, GH68, GH88, GH105, AA4, AA10, and PL8, indicating its polysaccharide catabolic potential. Genome-wide analysis identified one Lytic Polysaccharide Monooxygenase (LPMO) gene involved in oxidative polysaccharide cleavage. LPMOs co-localized with chitin-active glycoside hydrolase GH18, along with its association with cellulose enzymes. Protein-protein interaction and gene networking studies revealed LPMO co-occurrence and interaction with chitinase and cellulose degradation. Structural predictions provided LPMO substrate-binding sites, emphasizing their functional significance in biomass degradation. Functional annotation identified 121 key domains implicated in plant biomass degradation.</p> Conclusion <p>The present study elucidates the genomic organization of LPMO genes in <i>P. barengoltzii</i>, highlighting its potential for biomass hydrolysis. The genomic insights underscore the need for further experimental validation of <i>P. barengoltzii</i> to enhance ruminal fermentation and plant biomass decomposition.</p>

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Genome-wide characterization of LPMOs and CAZymes in camel rumen-derived Paenibacillus barengoltzii highlights strong lignocellulolytic potential

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月15日

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  • Shaswat Parmar,
  • Shivangi Patel,
  • Srinivas Duggirala,
  • Ashish Patel,
  • Ketankumar Panchal,
  • Subhash Jakhesara

摘要

Background

Camels exhibit remarkable adaptability to harsh environments, in part due to their unique ruminal microbiota. Paenibacillus barengoltzii, isolated from camel rumen, plays a crucial role in rumen fermentation and plant biomass degradation. The present study aimed to identify fermentative genes and assess their lignocellulolytic potential.

Results

Whole-genome sequencing was performed on the Illumina MiSeq platform to enable comprehensive comparative genomic analysis of carbohydrate-active enzymes (CAZymes) across 11 Paenibacillus species. It was observed that species-specific CAZymes repertoires, with P. barengoltzii harboring genes for plant biomass degradation, including GH2, GH13, GH20, GH27, GH28, GH32, GH62, GH65, GH67, GH68, GH88, GH105, AA4, AA10, and PL8, indicating its polysaccharide catabolic potential. Genome-wide analysis identified one Lytic Polysaccharide Monooxygenase (LPMO) gene involved in oxidative polysaccharide cleavage. LPMOs co-localized with chitin-active glycoside hydrolase GH18, along with its association with cellulose enzymes. Protein-protein interaction and gene networking studies revealed LPMO co-occurrence and interaction with chitinase and cellulose degradation. Structural predictions provided LPMO substrate-binding sites, emphasizing their functional significance in biomass degradation. Functional annotation identified 121 key domains implicated in plant biomass degradation.

Conclusion

The present study elucidates the genomic organization of LPMO genes in P. barengoltzii, highlighting its potential for biomass hydrolysis. The genomic insights underscore the need for further experimental validation of P. barengoltzii to enhance ruminal fermentation and plant biomass decomposition.