Scholarlink

Background <p>Valorization of agri-food waste as nutrient-rich substrates provides a sustainable strategy for microbial bioprocessing. <i>Blakeslea trispora</i> is a well-known fungal producer of natural β-carotene; however, its performance on low-cost industrial waste streams remains underexplored.</p> Results <p>In this study, dairy sludge and soybean meal were evaluated as alternative carbon and nitrogen sources for β-carotene biosynthesis by <i>B. trispora</i>. Response Surface Methodology (RSM) identified an optimized medium containing 12% dairy sludge, 2% soybean meal, and 0.4% yeast extract, yielding 65&#xa0;g/L biomass and 352&#xa0;μg/mL β-carotene in laboratory-scale fermentation. The pigment extracted from optimized cultures was confirmed as β-carotene through HPLC, FTIR, and <sup>13</sup>C-NMR analyses. Biofunctional assays revealed antimicrobial activity (MIC: 250–500&#xa0;mg/mL), with greater susceptibility in Gram-positive strains. Moderate antioxidant activity was observed in the DPPH assay (38% inhibition), and cytotoxic evaluation against Caco-2 cells resulted in an IC₅₀ of 400&#xa0;mg/mL, indicating potential anticancer effects.</p> Conclusion <p>These findings demonstrate that agri-food wastes can serve as efficient substrates for fungal β-carotene production, supporting cost-effective and sustainable microbial bioprocessing. The functional properties of the extracted β-carotene further highlight its applicability as a bioactive microbial metabolite with antimicrobial, antioxidant, and anticancer potential.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sustainable waste-based fermentation for enhanced β-carotene biosynthesis by Blakeslea trispora and functional evaluation of the extracted pigment

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月30日

查看全文
  • Negin Ghazanfari,
  • Ali Motlagh Hosseini,
  • Fereshteh Falah,
  • Motahareh Nasiri,
  • Farideh Tabatabaei Yazdi,
  • Mohamad Reza Razavi,
  • Alireza Vasiee

摘要

Background

Valorization of agri-food waste as nutrient-rich substrates provides a sustainable strategy for microbial bioprocessing. Blakeslea trispora is a well-known fungal producer of natural β-carotene; however, its performance on low-cost industrial waste streams remains underexplored.

Results

In this study, dairy sludge and soybean meal were evaluated as alternative carbon and nitrogen sources for β-carotene biosynthesis by B. trispora. Response Surface Methodology (RSM) identified an optimized medium containing 12% dairy sludge, 2% soybean meal, and 0.4% yeast extract, yielding 65 g/L biomass and 352 μg/mL β-carotene in laboratory-scale fermentation. The pigment extracted from optimized cultures was confirmed as β-carotene through HPLC, FTIR, and 13C-NMR analyses. Biofunctional assays revealed antimicrobial activity (MIC: 250–500 mg/mL), with greater susceptibility in Gram-positive strains. Moderate antioxidant activity was observed in the DPPH assay (38% inhibition), and cytotoxic evaluation against Caco-2 cells resulted in an IC₅₀ of 400 mg/mL, indicating potential anticancer effects.

Conclusion

These findings demonstrate that agri-food wastes can serve as efficient substrates for fungal β-carotene production, supporting cost-effective and sustainable microbial bioprocessing. The functional properties of the extracted β-carotene further highlight its applicability as a bioactive microbial metabolite with antimicrobial, antioxidant, and anticancer potential.