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<p><i>Ornithinibacillus liuyingii</i> sp. nov. was proposed, with type strain 27F3<sup>T</sup> (= GDMCC 1.5626<sup>T</sup> = JCM 38003<sup>T</sup>) isolated from the lung of <i>Apodemus chevrieri</i> collected on the Guizhou Plateau, China. Phylogenetic distinctiveness from its closest relative, <i>Ornithinibacillus scapharcae</i> TW25<sup>T</sup>, was confirmed by 98.5% 16S rRNA gene similarity, 76.98% average nucleotide identity, and 21.9% digital DNA–DNA hybridization value. Phenotypically, strain 27F3<sup>T</sup> was characterized as a motile, rod-shaped bacterium exhibiting a Gram-stain-negative reaction, an atypical feature within the otherwise Gram-positive genus <i>Ornithinibacillus</i>. The strain formed greyish-light purple colonies and displayed a distinct antimicrobial resistance profile, including resistance to cefepime, polymyxin E, and nalidixic acid. Chemotaxonomic analysis revealed iso-C<sub>15:0</sub>, anteiso-C<sub>15:0</sub>, and iso-C<sub>17:0</sub> as major fatty acids and menaquinone MK-7 as the predominant respiratory quinone. Genomic analysis identified genes associated with sporulation and oxidative stress defense, and numerous homologs of virulence-associated factors were detected. However, 16S rRNA gene amplicon sequencing of 90 additional rodent lungs did not detect close relatives (max identity 95.8%), suggesting that <i>O. liuyingii</i> should not be a common colonizer. This study describes the <i>Ornithinibacillus</i> species isolated from a mammalian respiratory tract and highlights its atypical Gram-stain reaction, which may contributing to our understanding into the diversity of the genus. The discovery may underscore the hidden complexity within wildlife-associated microbiota and emphasizes the importance of pathogen surveillance in a One Health context.</p>

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Ornithinibacillus liuyingii sp. nov.: a novel multidrug-resistant and potentially pathogenic bacterium isolated from Apodemus chevrieri lung tissue

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月23日

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  • Jian Zhou,
  • Tao Gu,
  • Ya Zhang,
  • Jingzhu Zhou,
  • Xiaoxiao Zeng,
  • Fengming Chen,
  • Yong Hu,
  • Shijun Li

摘要

Ornithinibacillus liuyingii sp. nov. was proposed, with type strain 27F3T (= GDMCC 1.5626T = JCM 38003T) isolated from the lung of Apodemus chevrieri collected on the Guizhou Plateau, China. Phylogenetic distinctiveness from its closest relative, Ornithinibacillus scapharcae TW25T, was confirmed by 98.5% 16S rRNA gene similarity, 76.98% average nucleotide identity, and 21.9% digital DNA–DNA hybridization value. Phenotypically, strain 27F3T was characterized as a motile, rod-shaped bacterium exhibiting a Gram-stain-negative reaction, an atypical feature within the otherwise Gram-positive genus Ornithinibacillus. The strain formed greyish-light purple colonies and displayed a distinct antimicrobial resistance profile, including resistance to cefepime, polymyxin E, and nalidixic acid. Chemotaxonomic analysis revealed iso-C15:0, anteiso-C15:0, and iso-C17:0 as major fatty acids and menaquinone MK-7 as the predominant respiratory quinone. Genomic analysis identified genes associated with sporulation and oxidative stress defense, and numerous homologs of virulence-associated factors were detected. However, 16S rRNA gene amplicon sequencing of 90 additional rodent lungs did not detect close relatives (max identity 95.8%), suggesting that O. liuyingii should not be a common colonizer. This study describes the Ornithinibacillus species isolated from a mammalian respiratory tract and highlights its atypical Gram-stain reaction, which may contributing to our understanding into the diversity of the genus. The discovery may underscore the hidden complexity within wildlife-associated microbiota and emphasizes the importance of pathogen surveillance in a One Health context.