Scholarlink

<p>Marine microorganisms residing in the Red Sea have been recognized as valuable sources of novel natural products with potential medical applications. One distinct bacterial isolate coded MMM3 was isolated from the marine tunicate <i>Phallusia nigra</i>, gathered from the Egyptian coast of the Red Sea. The present study aimed to identify and assess the isolated strain’s biological activities. After genomic extraction and PCR amplification, the bacterial strain was identified through 16&#xa0;S rRNA gene sequencing as <i>Heyndrickxia ginsengihumi</i> and deposited in (NCBI) GenBank database under the accession code PX237387. The tested isolate was cultivated in R2A liquid medium for ten days at 25&#xa0;°C in a shaking incubator set at 220&#xa0;rpm. The crude extract exhibited effective COX-2 inhibition and antioxidant activity with IC₅₀ value of 107.07 and 451.75&#xa0;µg/ml, respectively. The crude extract exerted antifungal effect (inhibition zone of 9&#xa0;mm) against <i>Candida albicans</i> ATCC 10,231. In addition, the crude extract demonstrated significant cytotoxic activities against HepG2, A-549, MCF-7 and HCT-116 cell lines with IC<sub>50</sub> values of 54.67 ± 1.05, 60.19 ± 1.23, 108.79 ± 2.08, and 73.57 ± 1.48&#xa0;µg/mL, respectively. Regarding the chemical characterization of the crude extract, LC-MS analysis indicated diverse secondary metabolites including linoleic acid, myristoyl glucose-3-phosphate, and 2-acetoxy-4-pentadecylbenzoic acid. The identified metabolites suggest a synergistic contribution to the biological activities of the isolated strain, highlighting its potential as a source of bioactive compounds. The results indicated that the bacteria isolated from Red Sea coast is a promising source of bioactive molecules with medical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular identification and biological activity of a red sea bacterial isolate

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月25日

查看全文
  • Mohamed M. Matar,
  • Yomna N. Elkholy,
  • Walaa A. Negm,
  • Hossam H. Elfeky,
  • Mahmoud A. Yassien

摘要

Marine microorganisms residing in the Red Sea have been recognized as valuable sources of novel natural products with potential medical applications. One distinct bacterial isolate coded MMM3 was isolated from the marine tunicate Phallusia nigra, gathered from the Egyptian coast of the Red Sea. The present study aimed to identify and assess the isolated strain’s biological activities. After genomic extraction and PCR amplification, the bacterial strain was identified through 16 S rRNA gene sequencing as Heyndrickxia ginsengihumi and deposited in (NCBI) GenBank database under the accession code PX237387. The tested isolate was cultivated in R2A liquid medium for ten days at 25 °C in a shaking incubator set at 220 rpm. The crude extract exhibited effective COX-2 inhibition and antioxidant activity with IC₅₀ value of 107.07 and 451.75 µg/ml, respectively. The crude extract exerted antifungal effect (inhibition zone of 9 mm) against Candida albicans ATCC 10,231. In addition, the crude extract demonstrated significant cytotoxic activities against HepG2, A-549, MCF-7 and HCT-116 cell lines with IC50 values of 54.67 ± 1.05, 60.19 ± 1.23, 108.79 ± 2.08, and 73.57 ± 1.48 µg/mL, respectively. Regarding the chemical characterization of the crude extract, LC-MS analysis indicated diverse secondary metabolites including linoleic acid, myristoyl glucose-3-phosphate, and 2-acetoxy-4-pentadecylbenzoic acid. The identified metabolites suggest a synergistic contribution to the biological activities of the isolated strain, highlighting its potential as a source of bioactive compounds. The results indicated that the bacteria isolated from Red Sea coast is a promising source of bioactive molecules with medical applications.