Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have emerged as a major global public health threat. Cefiderocol is a novel siderophore-conjugated cephalosporin with potent activity to treat severe infections caused by CRKP. In this study, we investigated the underlying mechanisms leading to cefiderocol resistance in an ST11-KL64 hypervirulent K. pneumoniae FK3023 isolated from a patient with infective fever and chronic kidney disease in China. Methods Whole genome sequencing (WGS), amino acid sequences alignment, cirA gene complementation and lactamases genes cloning assays were performed to elucidate the molecular basis of cefiderocol resistance. Pathogenicity was evaluated by quantitative siderophore production, string test, capsule quantification, and a Galleria mellonella infection model. Results This strain carried a hybrid plasmid that contained both virulence and resistance determinants, including key virulence factors (iutA-iucABCD operon and rmpA gene) and β-lactamases genes (blaKPC−2, blaTEM−1B, blaCTX−M−3, blaCTX−M−65). Compared to the wild-type siderophore receptor CirA, K. pneumoniae FK3023 carried 119 nucleotide deletion (c.1830_1948del), leading to a frameshift, followed by a premature stop codon (Val611TyrfsTer32), resulting in a novel mutation in CirA. K. pneumoniae FK3023 complemented with the wild-type cirA showed a substantial decrease in cefiderocol MIC, further confirming the function of CirA. Each of the six identified β-lactamases genes in K. pneumoniae FK3023 was cloned into a pUC vector, and then expressed in Escherichia coli cells. The E. coli strains carrying blaSHV−12 showed an increase in cefiderocol MIC compared to the E. coli strains carrying empty vectors. Conclusions In conclusion, we described a clinical ST11-KL64 hypervirulent K. pneumoniae isolate exhibiting cefiderocol resistance likely mediated by a novel CirA truncation and blaSHV−12. K. pneumoniae FK3023 also carried the hybrid plasmid and two additional resistance plasmids, highlighting the convergence of hypervirulence and multidrug resistance and underscoring the challenges for clinical management.

Cefiderocol resistance in a clinical ST11-KL64 hypervirulent Klebsiella pneumoniae isolate mediated by a novel CirA truncation and SHV-12 β-lactamase

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2026年3月6日

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Peiyao Zhou,
Jiana Fu,
Xiaoxia He,
Haojin Gao,
Chunyang Wu,
Fangyou Yu,
Ying Zhou

摘要

Background

Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have emerged as a major global public health threat. Cefiderocol is a novel siderophore-conjugated cephalosporin with potent activity to treat severe infections caused by CRKP. In this study, we investigated the underlying mechanisms leading to cefiderocol resistance in an ST11-KL64 hypervirulent K. pneumoniae FK3023 isolated from a patient with infective fever and chronic kidney disease in China.

Methods

Whole genome sequencing (WGS), amino acid sequences alignment, cirA gene complementation and lactamases genes cloning assays were performed to elucidate the molecular basis of cefiderocol resistance. Pathogenicity was evaluated by quantitative siderophore production, string test, capsule quantification, and a Galleria mellonella infection model.

Results

This strain carried a hybrid plasmid that contained both virulence and resistance determinants, including key virulence factors (iutA-iucABCD operon and rmpA gene) and β-lactamases genes (bla_KPC−2, bla_TEM−1B, bla_CTX−M−3, bla_CTX−M−65). Compared to the wild-type siderophore receptor CirA, K. pneumoniae FK3023 carried 119 nucleotide deletion (c.1830_1948del), leading to a frameshift, followed by a premature stop codon (Val611TyrfsTer32), resulting in a novel mutation in CirA. K. pneumoniae FK3023 complemented with the wild-type cirA showed a substantial decrease in cefiderocol MIC, further confirming the function of CirA. Each of the six identified β-lactamases genes in K. pneumoniae FK3023 was cloned into a pUC vector, and then expressed in Escherichia coli cells. The E. coli strains carrying bla_SHV−12 showed an increase in cefiderocol MIC compared to the E. coli strains carrying empty vectors.

Conclusions

In conclusion, we described a clinical ST11-KL64 hypervirulent K. pneumoniae isolate exhibiting cefiderocol resistance likely mediated by a novel CirA truncation and bla_SHV−12. K. pneumoniae FK3023 also carried the hybrid plasmid and two additional resistance plasmids, highlighting the convergence of hypervirulence and multidrug resistance and underscoring the challenges for clinical management.