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<p>Colorectal cancer (CRC) remains a major global health burden as one of the leading causes of cancer-related mortality. Recent research has highlighted the crucial role of gut microbiota in CRC development. Through high-throughput full-length <i>16&#xa0;S rDNA</i> sequencing of tumor and adjacent non-tumor tissues from 14 CRC patients, significant microbial differences were identified. At the phylum level, Firmicutes (52.59%), Bacteroidetes (18.51%), and Proteobacteria (14.89%) dominated both tissue types, while at the genus level, <i>Bacteroides</i> (8.02%) and <i>Escherichia</i> (4.50%) showed the highest abundance. Notably, 17 bacterial species exhibited differential abundance between tumor and normal tissues, with <i>Anaerotignum faecicola</i> and <i>Pseudomonas fluorescens</i> being significantly enriched in tumor tissues. Functional prediction analysis revealed the microbiota’s predominant involvement in carbohydrate metabolism, amino acid metabolism, and energy metabolism pathways. Subsequent validation in 20 additional patient samples confirmed <i>P. fluorescens</i> enrichment in tumor tissues, and in vitro experiments demonstrated its ability to promote CRC cell viability and proliferation. These findings provide valuable insights into CRC-associated microbial signatures and suggest <i>P. fluorescens</i> as a potential contributor to tumor progression, offering new directions for developing diagnostic markers and therapeutic interventions in CRC management.</p> Graphical Abstract <p></p>

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Identification and functional characterization of Pseudomonas fluorescens as a novel intratumoral bacterium in colorectal cancer

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月24日

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  • Qiliu Qian,
  • Nan Li,
  • Suna Cha,
  • Shiya Zheng,
  • Wenhao Li,
  • Guangxuan Yin,
  • Mingjun Sun,
  • Ping Ye,
  • Mingyue Hu,
  • Ruihua Shi,
  • Yongqi Zhang,
  • Weitao Shen

摘要

Colorectal cancer (CRC) remains a major global health burden as one of the leading causes of cancer-related mortality. Recent research has highlighted the crucial role of gut microbiota in CRC development. Through high-throughput full-length 16 S rDNA sequencing of tumor and adjacent non-tumor tissues from 14 CRC patients, significant microbial differences were identified. At the phylum level, Firmicutes (52.59%), Bacteroidetes (18.51%), and Proteobacteria (14.89%) dominated both tissue types, while at the genus level, Bacteroides (8.02%) and Escherichia (4.50%) showed the highest abundance. Notably, 17 bacterial species exhibited differential abundance between tumor and normal tissues, with Anaerotignum faecicola and Pseudomonas fluorescens being significantly enriched in tumor tissues. Functional prediction analysis revealed the microbiota’s predominant involvement in carbohydrate metabolism, amino acid metabolism, and energy metabolism pathways. Subsequent validation in 20 additional patient samples confirmed P. fluorescens enrichment in tumor tissues, and in vitro experiments demonstrated its ability to promote CRC cell viability and proliferation. These findings provide valuable insights into CRC-associated microbial signatures and suggest P. fluorescens as a potential contributor to tumor progression, offering new directions for developing diagnostic markers and therapeutic interventions in CRC management.

Graphical Abstract