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Background <p>The nasal cavity serves as a primary contact site and is a common location for colonization by symbiotic, opportunistic, and potentially pathogenic bacteria. Diabetic patients are more susceptible to colonization by opportunistic microorganisms due to impaired immune function, altered normal flora, and increased exposure to healthcare. This study aimed to investigate the nasal colonization of Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Enterobacteriaceae</i>) bacteria in diabetic and non-diabetic individuals, assessing phenotypic traits including antibiotic resistance and biofilm production, as well as investigating the presence of resistant genes.</p> Materials and methods <p>In this cross-sectional study, nasal swabs were collected from 150 diabetic and 150 non-diabetic individuals. Isolates were identified and evaluated phenotypically (Antibiotic resistance using the disk diffusion method and biofilm formation by the microtiter plate method) and genotypically (resistance genes including <i>mecA</i>, <i>bla</i><sub>CTX</sub>, <i>bla</i><sub>SHV</sub>, and <i>bla</i><sub>TEM</sub>) by PCR.</p> Results <p>The rate of <i>S. aureus</i> colonization was higher in diabetics (18.7%) than in non-diabetics (12.7%) and MRSA colonization was significantly higher in diabetics (8% vs. 1.3%). High antibiotic resistance was not observed except for tetracycline (nearly 50%) in <i>S. aureus</i> isolates from both groups. There was no statistically significant difference in the occurrence of MDR <i>S. aureus</i> between the diabetic (32.1%) and non-diabetic (31.6%) groups. <i>Enterobacteriaceae</i> colonization was 3.3% in diabetics and 7.3% in non-diabetics. Although none were phenotypically ESBL-positive, <i>bla</i><sub>CTX</sub>, <i>bla</i><sub>TEM</sub>, <i>and bla</i><sub>SHV</sub> genes were present in about 40% of the isolates.</p> Conclusion <p>Nasal MRSA colonization was more common among diabetic patients than non-diabetics. The findings of this study highlight the need for ongoing monitoring of nasal colonization of MRSA in different populations and settings, which may lead to the development of effective preventive and therapeutic strategies to control infections caused by nasal colonization.</p>

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The spectrum of nasal colonization: frequency and resistant patterns in diabetes versus non-diabetes population

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月4日

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  • Maryam Rabeh,
  • Samaneh Shahrokh,
  • Mojtaba Akbari,
  • Najmeh Ansari,
  • Mansour Siavash,
  • Maryam Yazdi

摘要

Background

The nasal cavity serves as a primary contact site and is a common location for colonization by symbiotic, opportunistic, and potentially pathogenic bacteria. Diabetic patients are more susceptible to colonization by opportunistic microorganisms due to impaired immune function, altered normal flora, and increased exposure to healthcare. This study aimed to investigate the nasal colonization of Gram-positive (Staphylococcus aureus) and Gram-negative (Enterobacteriaceae) bacteria in diabetic and non-diabetic individuals, assessing phenotypic traits including antibiotic resistance and biofilm production, as well as investigating the presence of resistant genes.

Materials and methods

In this cross-sectional study, nasal swabs were collected from 150 diabetic and 150 non-diabetic individuals. Isolates were identified and evaluated phenotypically (Antibiotic resistance using the disk diffusion method and biofilm formation by the microtiter plate method) and genotypically (resistance genes including mecA, blaCTX, blaSHV, and blaTEM) by PCR.

Results

The rate of S. aureus colonization was higher in diabetics (18.7%) than in non-diabetics (12.7%) and MRSA colonization was significantly higher in diabetics (8% vs. 1.3%). High antibiotic resistance was not observed except for tetracycline (nearly 50%) in S. aureus isolates from both groups. There was no statistically significant difference in the occurrence of MDR S. aureus between the diabetic (32.1%) and non-diabetic (31.6%) groups. Enterobacteriaceae colonization was 3.3% in diabetics and 7.3% in non-diabetics. Although none were phenotypically ESBL-positive, blaCTX, blaTEM, and blaSHV genes were present in about 40% of the isolates.

Conclusion

Nasal MRSA colonization was more common among diabetic patients than non-diabetics. The findings of this study highlight the need for ongoing monitoring of nasal colonization of MRSA in different populations and settings, which may lead to the development of effective preventive and therapeutic strategies to control infections caused by nasal colonization.