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<p>The microbial diversity found in the areas contaminated by industrial waste is a great place to drain the tough enzymes that are possible to use for the biotechnological industry. In this study, bacteria were taken from different places in the Kars Sugar Factory. Then, the bacteria were subjected to extensive morphological, biochemical, and molecular examinations. The samples were able to grow at pH of 5.0 to 9.0 and in temperature ranging from 40 to 85&#xa0;°C. The examination showed that most of the strains belonged to the genera <i>Bacillus</i>, <i>Geobacillus</i>, <i>Klebsiella</i>, and <i>Acinetobacter</i>. High frequency of hydrolase production was among the isolates. 60.6% of the isolates were identified as lipase producers, 66.7% as amylase producers, 83.3% as xylanase producers, and 89.4% as protease producers. The greatest part of isolates could produce more than one enzyme at the same time. These results are a further argument in favor of the industrial application of microbial enzymes in waste utilization and biofuel production. The Kars Sugar Factory is a microbially rich niche of great importance.</p>

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Molecular characterization, identification and enzyme-producing potential of thermophilic bacterial diversity isolated from the Kars sugar factory

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月28日

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  • Ünal Alkaşi,
  • Orhan Uluçay

摘要

The microbial diversity found in the areas contaminated by industrial waste is a great place to drain the tough enzymes that are possible to use for the biotechnological industry. In this study, bacteria were taken from different places in the Kars Sugar Factory. Then, the bacteria were subjected to extensive morphological, biochemical, and molecular examinations. The samples were able to grow at pH of 5.0 to 9.0 and in temperature ranging from 40 to 85 °C. The examination showed that most of the strains belonged to the genera Bacillus, Geobacillus, Klebsiella, and Acinetobacter. High frequency of hydrolase production was among the isolates. 60.6% of the isolates were identified as lipase producers, 66.7% as amylase producers, 83.3% as xylanase producers, and 89.4% as protease producers. The greatest part of isolates could produce more than one enzyme at the same time. These results are a further argument in favor of the industrial application of microbial enzymes in waste utilization and biofuel production. The Kars Sugar Factory is a microbially rich niche of great importance.