Scholarlink

<p>A novel Gram-stain-negative, facultatively anaerobic, endospore-forming bacterium, designated DYY-L-2<sup>T</sup>, was isolated from the saliva of a 69-year-old patient with chronic periodontitis in Hengyang, China. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain DYY-L-2<sup>T</sup> belonged to the genus <i>Paenibacillus</i>, with the highest similarity to <i>Paenibacillus konsidensis</i> LBY<sup>T</sup> (98.6%), followed by <i>Paenibacillus vini</i> LAM0504<sup>T</sup> (97.2%). Whole-genome sequencing yielded a complete circular chromosome of 5,642,305&#xa0;bp with a genomic DNA G + C content of 50.8%. Overall genome relatedness index analysis indicated low average nucleotide identity (ANI &lt; 80%) and in silico DNA–DNA hybridization (isDDH &lt; 25%) values between strain DYY-L-2<sup>T</sup> and its closely related type strains, supporting its status as a novel genospecies. The strain grew optimally at 37&#xa0;°C, in pH 7.0, and tolerated up to 5% (w/v) NaCl. Major cellular fatty acids were C<sub>16:0</sub> and anteiso-C<sub>15:0</sub>. The genomic functional annotation identified unique regions enriched with mobile genetic elements and Type I restriction-modification systems (e.g. <i>hsdR</i> and <i>hsdM</i>), suggesting adaptive mechanisms for genomic stability in the oral environment. Based on polyphasic taxonomic data, strain DYY-L-2<sup>T</sup> represents a novel species within the genus <i>Paenibacillus</i>, for which the name <i>Paenibacillus salivarius</i> sp. nov. is proposed. The type strain is DYY-L-2<sup>T</sup> (= GDMCC 1.6010<sup>T</sup> = KCTC 43851<sup>T</sup>). This study expands the understanding of the <i>Paenibacillus</i> diversity in host-associated environments and provides insights into its ecological role in the human oral microbiome.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phenotypic and genomic characterization of Paenibacillus salivarius sp. nov., isolated from the human oral cavity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月13日

查看全文
  • Xin Huang,
  • Ming Chen,
  • Ping Huang,
  • Qi Zhou,
  • Zhangjun Ling,
  • Yan Wu,
  • Dan Mu,
  • Long Xu,
  • Yao Peng,
  • Xunchao Cai

摘要

A novel Gram-stain-negative, facultatively anaerobic, endospore-forming bacterium, designated DYY-L-2T, was isolated from the saliva of a 69-year-old patient with chronic periodontitis in Hengyang, China. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain DYY-L-2T belonged to the genus Paenibacillus, with the highest similarity to Paenibacillus konsidensis LBYT (98.6%), followed by Paenibacillus vini LAM0504T (97.2%). Whole-genome sequencing yielded a complete circular chromosome of 5,642,305 bp with a genomic DNA G + C content of 50.8%. Overall genome relatedness index analysis indicated low average nucleotide identity (ANI < 80%) and in silico DNA–DNA hybridization (isDDH < 25%) values between strain DYY-L-2T and its closely related type strains, supporting its status as a novel genospecies. The strain grew optimally at 37 °C, in pH 7.0, and tolerated up to 5% (w/v) NaCl. Major cellular fatty acids were C16:0 and anteiso-C15:0. The genomic functional annotation identified unique regions enriched with mobile genetic elements and Type I restriction-modification systems (e.g. hsdR and hsdM), suggesting adaptive mechanisms for genomic stability in the oral environment. Based on polyphasic taxonomic data, strain DYY-L-2T represents a novel species within the genus Paenibacillus, for which the name Paenibacillus salivarius sp. nov. is proposed. The type strain is DYY-L-2T (= GDMCC 1.6010T = KCTC 43851T). This study expands the understanding of the Paenibacillus diversity in host-associated environments and provides insights into its ecological role in the human oral microbiome.