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Background <p>To investigate the mechanism by which <i>Acinetobacter lwoffii</i>-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN).</p> Methods <p>After establishing a co-culture model of <i>Acinetobacter lwoffii</i> and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR).</p> Results <p>Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (<i>P</i> &lt; 0.05). The optimal coculture model with <i>Acinetobacter lwoffii</i> was an MOI of 100 following 18&#xa0;h-24&#xa0;h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12&#xa0;h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12&#xa0;h to 24&#xa0;h (all <i>P</i> &lt; 0.05).</p> Conclusion <p><i>Acinetobacter lwoffii</i> can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner.</p>

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The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1

  • 期刊论文

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  • 发表日期：

    2025年5月26日

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  • Jiayuan Du,
  • Tianmeng Tan,
  • Yaojie Yu,
  • He Wang

摘要

Background

To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN).

Methods

After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR).

Results

Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (P < 0.05). The optimal coculture model with Acinetobacter lwoffii was an MOI of 100 following 18 h-24 h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12 h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12 h to 24 h (all P < 0.05).

Conclusion

Acinetobacter lwoffii can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner.